A1 Refereed original research article in a scientific journal
Adherence of Candida albicans to the surface of polymethylmethacrylate- E glass fiber composite used in dentures.
Authors: Waltimo T, Tanner J, Vallittu PK, Haapasalo M
Publisher: Quintessence Publishing
Publication year: 1999
Volume: 12
Issue: 1
First page : 83
Last page: 86
Abstract
AbstractPURPOSE: The
use of reinforcing fibers in dentures has raised concerns about
possible increased adherence of yeasts to the surface. The aim of this
in vitro study was to compare the adherence of Candida albicans to the
surface of denture-base polymer and to E-glass fibers.MATERIALS AND METHODS: Test
specimens were made from an autopolymerized denture-base resin
(Palapress) reinforced with preimpregnated unidirectional E-glass
fibers, which were exposed at the surface. The test specimens were
pretreated with parotid saliva and incubated without agitation in
standardized yeast suspensions (10(8) colony-forming units per mL) in
phosphate-buffered saline at 37 degrees C for 1 hour. The test specimens
were then washed to remove nonadherent cells. After being air dried,
they were sputter coated with gold-palladium for scanning electron
microscopy (SEM). To compare the adherence to different surfaces, the
number of yeast cells found either on the polymer matrix or on the glass
fibers was counted from SEM fields (170 microns x 120 microns, 600 x)
of randomly selected areas.RESULTS: The mean density of
yeast cell found on the surface of the polymer matrix was significantly
higher (P < 0.001) than that on the surface of glass fibers. The
number of adherent yeast cells found at the interface between the fibers
and polymer matrix was high.CONCLUSION: The adherence
of C albicans to E-glass fibers was lower than to polymer matrix in the
denture composite. If fibers are exposed only during polishing of the
composite, the reinforcing material appears not to increase the
adherence of this common oral yeast. However, areas with permanently
exposed fibers may provide mechanical retention for yeast cells at the
interface of the components.
AbstractPURPOSE: The
use of reinforcing fibers in dentures has raised concerns about
possible increased adherence of yeasts to the surface. The aim of this
in vitro study was to compare the adherence of Candida albicans to the
surface of denture-base polymer and to E-glass fibers.MATERIALS AND METHODS: Test
specimens were made from an autopolymerized denture-base resin
(Palapress) reinforced with preimpregnated unidirectional E-glass
fibers, which were exposed at the surface. The test specimens were
pretreated with parotid saliva and incubated without agitation in
standardized yeast suspensions (10(8) colony-forming units per mL) in
phosphate-buffered saline at 37 degrees C for 1 hour. The test specimens
were then washed to remove nonadherent cells. After being air dried,
they were sputter coated with gold-palladium for scanning electron
microscopy (SEM). To compare the adherence to different surfaces, the
number of yeast cells found either on the polymer matrix or on the glass
fibers was counted from SEM fields (170 microns x 120 microns, 600 x)
of randomly selected areas.RESULTS: The mean density of
yeast cell found on the surface of the polymer matrix was significantly
higher (P < 0.001) than that on the surface of glass fibers. The
number of adherent yeast cells found at the interface between the fibers
and polymer matrix was high.CONCLUSION: The adherence
of C albicans to E-glass fibers was lower than to polymer matrix in the
denture composite. If fibers are exposed only during polishing of the
composite, the reinforcing material appears not to increase the
adherence of this common oral yeast. However, areas with permanently
exposed fibers may provide mechanical retention for yeast cells at the
interface of the components.
UTU Research Portal