A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
New Types of Flavonol Oligoglycosides Accumulate in the Hemolymph of Birch-Feeding Sawfly Larvae
Tekijät: Vihakas MA, Kapari L, Salminen JP
Kustantaja: SPRINGER
Julkaisuvuosi: 2010
Journal: Journal of Chemical Ecology
Tietokannassa oleva lehden nimi: JOURNAL OF CHEMICAL ECOLOGY
Lehden akronyymi: J CHEM ECOL
Numero sarjassa: 8
Vuosikerta: 36
Numero: 8
Aloitussivu: 864
Lopetussivu: 872
Sivujen määrä: 9
ISSN: 0098-0331
DOI: https://doi.org/10.1007/s10886-010-9822-2
Tiivistelmä
Larvae of nine species of sawflies (Symphyta) were fed with the foliage of three birch species, after which the larval hemolymph composition was studied by HPLC-DAD and HPLC-ESI-MS. The hemolymph of sawfly larvae contained high concentrations of flavonol oligoglycosides (tri-, tetra-, penta-, and hexaglycosides) that could not be found in the larval foliar diet. In addition, there were significant between-sawfly species differences in both flavonoid composition and concentration (from 0.6 to 12.3 mg/ml) of the hemolymph. This suggested that the studied species have different biosynthetic activities for the synthesis of flavonoid oligoglycosides. Variation in the foliar diets did not cause differences in the hemolymph composition. Our hypothesis is that sawflies use foliar flavonoid monoglycosides rather than flavonoid aglycones to produce these new types of oligoglycosides. These findings open up new possibilities for understanding the more holistic role of flavonoids in insect biochemistry and complex interactions between plants and herbivores.
Larvae of nine species of sawflies (Symphyta) were fed with the foliage of three birch species, after which the larval hemolymph composition was studied by HPLC-DAD and HPLC-ESI-MS. The hemolymph of sawfly larvae contained high concentrations of flavonol oligoglycosides (tri-, tetra-, penta-, and hexaglycosides) that could not be found in the larval foliar diet. In addition, there were significant between-sawfly species differences in both flavonoid composition and concentration (from 0.6 to 12.3 mg/ml) of the hemolymph. This suggested that the studied species have different biosynthetic activities for the synthesis of flavonoid oligoglycosides. Variation in the foliar diets did not cause differences in the hemolymph composition. Our hypothesis is that sawflies use foliar flavonoid monoglycosides rather than flavonoid aglycones to produce these new types of oligoglycosides. These findings open up new possibilities for understanding the more holistic role of flavonoids in insect biochemistry and complex interactions between plants and herbivores.
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