A1 Refereed original research article in a scientific journal
Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue
Authors: Erami Z, Herrmann D, Warren SC, Nobis M, McGhee EJ, Lucas MC, Leung W, Reischmann N, Mrowinska A, Schwarz JP, Kadir S, Conway JRW, Vennin C, Karim SA, Campbell AD, Magenau A, Gallego-Ortega D, Ridgway RA, Murphy KJ, Walters SN, Law AM, Croucher DR, Grey ST, Herzog H, Zhang L, Gunning PW, Hardeman EC, Evans TRJ, Ormandy CJ, Sansom OJ, Strathdee D, Timpson P, Morton JP, Anderson KI
Publication year: 2016
Journal: Cell Reports
Journal name in source: Cell reports
Journal acronym: Cell Rep
Volume: 14
Issue: 1
First page : 152
Last page: 167
Number of pages: 16
ISSN: 2211-1247
eISSN: 2211-1247
DOI: https://doi.org/10.1016/j.celrep.2015.12.020
E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53 or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments.
