A1 Refereed original research article in a scientific journal
Mutagen effects on rat seminiferous tubules in vitro: induction of meiotic micronuclei by adriamycin
Authors: Toppari J, Lähdetie J, Härkönen P, Eerola E, Parvinen M
Publication year: 1986
Journal: Mutation Research
Journal name in source: Mutation research
Journal acronym: Mutat Res
Volume: 171
Issue: 2-3
First page : 149
Last page: 56
Number of pages: 8
ISSN: 0027-5107
DOI: https://doi.org/10.1016/0165-1218(86)90048-0
Abstract
Mutagen effect on male germ cells can be analyzed by micronucleus induction during meiotic divisions. These can be followed in vitro by culturing seminiferous tubular segments from stages of the epithelial cycle that contain late pachytene and diakinetic primary spermatocytes. We studied the formation of micronuclei in this test system using adriamycin as a model mutagen. Micronuclei were induced in a dose-dependent manner at concentrations of 1-10 ng/ml that were far below the dose that caused morphologically or biochemically detectable cytotoxic effects. The meiotic micronucleus induction in vitro is a potentially sensitive test system of male germ cell mutagenesis.
Mutagen effect on male germ cells can be analyzed by micronucleus induction during meiotic divisions. These can be followed in vitro by culturing seminiferous tubular segments from stages of the epithelial cycle that contain late pachytene and diakinetic primary spermatocytes. We studied the formation of micronuclei in this test system using adriamycin as a model mutagen. Micronuclei were induced in a dose-dependent manner at concentrations of 1-10 ng/ml that were far below the dose that caused morphologically or biochemically detectable cytotoxic effects. The meiotic micronucleus induction in vitro is a potentially sensitive test system of male germ cell mutagenesis.
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