A1 Refereed original research article in a scientific journal
Nucleotide sequence of black currant reversion associated nepovirus RNA1
Authors: Pacot-Hiriart C, Latvala-Kilby S, Lehto K
Publisher: ELSEVIER SCIENCE BV
Publication year: 2001
Journal:: Virus Research
Journal name in source: VIRUS RESEARCH
Journal acronym: VIRUS RES
Volume: 79
Issue: 1-2
First page : 145
Last page: 152
Number of pages: 8
ISSN: 0168-1702
DOI: https://doi.org/10.1016/S0168-1702(01)00342-2
Abstract
The RNA1 of black currant reversion associated nepovirus (BRAV) is 7711 nucleotides (nt) long, excluding the poly-A tail, and contains one long open reading frame (ORF) which is translated into a polyprotein of 2094 amino acids. The 5 ' NTR of BRAV RNA1 is 66 nt long and 78% identical with RNA2 5 ' NTR only over the first 57 nucleotides. The 3 ' non-translated region (3 ' NTR) is 1360 nucleotides long, and after the first 24 nucleotides 95% identical with the 3 ' NTR of RNA2. RNA1 3 ' NTR contains several stretches, 694-24 nucleotides in length, which are 60-80% similar to corresponding areas of the other viruses of the subgroup c of nepoviruses (BLMV, CLRV, PRMV or TomRSV). The 2094 amino acids-long polypeptide encoded by BRAV RNA1 is 33% identical with that of PRMV between amino acids 9 and 2057, and has significant similarity also to those of other nepoviruses and comoviruses. Conserved amino acid motifs, characteristic for the viral protease co-factor, the NTP-binding protein, the cysteine protease and the RdRp core domains, known to occur in the polyproteins of different viruses of the picornavirus-like supergroup, are all detected in the amino acid sequences encoded by BRAV RNA1. (C) 2001 Elsevier Science BN. All rights reserved.
The RNA1 of black currant reversion associated nepovirus (BRAV) is 7711 nucleotides (nt) long, excluding the poly-A tail, and contains one long open reading frame (ORF) which is translated into a polyprotein of 2094 amino acids. The 5 ' NTR of BRAV RNA1 is 66 nt long and 78% identical with RNA2 5 ' NTR only over the first 57 nucleotides. The 3 ' non-translated region (3 ' NTR) is 1360 nucleotides long, and after the first 24 nucleotides 95% identical with the 3 ' NTR of RNA2. RNA1 3 ' NTR contains several stretches, 694-24 nucleotides in length, which are 60-80% similar to corresponding areas of the other viruses of the subgroup c of nepoviruses (BLMV, CLRV, PRMV or TomRSV). The 2094 amino acids-long polypeptide encoded by BRAV RNA1 is 33% identical with that of PRMV between amino acids 9 and 2057, and has significant similarity also to those of other nepoviruses and comoviruses. Conserved amino acid motifs, characteristic for the viral protease co-factor, the NTP-binding protein, the cysteine protease and the RdRp core domains, known to occur in the polyproteins of different viruses of the picornavirus-like supergroup, are all detected in the amino acid sequences encoded by BRAV RNA1. (C) 2001 Elsevier Science BN. All rights reserved.
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