A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Identification of different Cecidophyopsis mites on Ribes in Finland
Tekijät: Lemmetty A, Tikkanen M, Tuovinen T, Lehto K
Julkaisuvuosi: 2004
Lehti:: Acta Horticulturae
Tietokannassa oleva lehden nimi: PROCEEDINGS OF THE TENTH INTERNATIONAL SYMPOSIUM ON SMALL FRUIT VIRUS DISEASES
Lehden akronyymi: ACTA HORTIC
Numero: 656
Aloitussivu: 115
Lopetussivu: 118
Sivujen määrä: 4
ISBN: 90-6605-099-3
ISSN: 0567-7572
DOI: https://doi.org/10.17660/ActaHortic.2004.656.17
Tiivistelmä
The black currant gall mite, Cecidophyopsis ribis, is a severe pest of black currant and also the vector of reversion disease. There are also other host-specific eriophyid mite species known to infest other Ribes species, for instance C. selachodon colonizes red currants and C. alpina mountain currants, and C. spicata both red and black currants. It is possible that different eriophyid species function as vectors of reversion disease, particularly in the alternative Ribes host species. We have used the multiplex PCR technique, based on the comparison of size differences of specific PCR amplified fragments of the mite rDNA, to identify the mites occurring in different Ribes species in Finland. Analysis of 118 mite samples from black currants revealed C. spicata in most (101) of these samples. C. ribis was found in 13 samples, and an unidentified banding pattern was detected in four mites samples from central Finland. PCR fragments obtained from several black currant mites were sequenced to confirm the result. Ten samples from red currants revealed the presence of a C. spicata/C. selachodon complex which could not be separated without sequencing. 16 mite samples from mountain currants revealed C alpina in one location, and a C spicata/C. selachodon complex in another location. All but one of the black currants analysed, and all red currants, were positive for Black currant reversion virus (BRV), as were also the mountain currants which were infested by the C. spicata/C. selachodon complex. These results indicate that in Finland C. spicata is the main vector of BRV in black currants, and may also be a vector of BRV in red currants and mountain currants.
The black currant gall mite, Cecidophyopsis ribis, is a severe pest of black currant and also the vector of reversion disease. There are also other host-specific eriophyid mite species known to infest other Ribes species, for instance C. selachodon colonizes red currants and C. alpina mountain currants, and C. spicata both red and black currants. It is possible that different eriophyid species function as vectors of reversion disease, particularly in the alternative Ribes host species. We have used the multiplex PCR technique, based on the comparison of size differences of specific PCR amplified fragments of the mite rDNA, to identify the mites occurring in different Ribes species in Finland. Analysis of 118 mite samples from black currants revealed C. spicata in most (101) of these samples. C. ribis was found in 13 samples, and an unidentified banding pattern was detected in four mites samples from central Finland. PCR fragments obtained from several black currant mites were sequenced to confirm the result. Ten samples from red currants revealed the presence of a C. spicata/C. selachodon complex which could not be separated without sequencing. 16 mite samples from mountain currants revealed C alpina in one location, and a C spicata/C. selachodon complex in another location. All but one of the black currants analysed, and all red currants, were positive for Black currant reversion virus (BRV), as were also the mountain currants which were infested by the C. spicata/C. selachodon complex. These results indicate that in Finland C. spicata is the main vector of BRV in black currants, and may also be a vector of BRV in red currants and mountain currants.
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