Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis

: Guzman C, Solman M, Abankwa D

: Lorenza Trabalzini, Saverio Francesco Retta

Publisher: Springer

: TOTOWA; 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA

: 2014

: Ras Signaling: Methods and Protocols

: Methods Mol.Biol.

: Methods in Molecular Biology

: 1120

: 307

: 326

: 20

: 978-1-62703-790-7

: 978-1-62703-791-4

: 1064-3745

DOI: https://doi.org/10.1007/978-1-62703-791-4_20

Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation Spectroscopy (RICS) are two powerful techniques to study the diffusion dynamics of fluorescently labeled proteins. FRAP and RICS can be easily applied on any commercial confocal microscope. In this chapter, we describe the principles of these methods and provide the reader with a detailed guide on how to apply these methods in the study of Ras nanoclustering and diffusion in the plasma membrane of live cells.