A3 Refereed book chapter or chapter in a compilation book
Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis
Authors: Guzman C, Solman M, Abankwa D
Editors: Lorenza Trabalzini, Saverio Francesco Retta
Publisher: HUMANA PRESS INC
Publishing place: TOTOWA; 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA
Publication year: 2014
Book title : Ras Signaling: Methods and Protocols
Journal name in source: Ras Signaling: Methods and Protocols
Journal acronym: Methods Mol.Biol.
Series title: Methods in Molecular Biology
Volume: 1120
First page : 307
Last page: 326
Number of pages: 20
ISBN: 978-1-62703-790-7
eISBN: 978-1-62703-791-4
ISSN: 1064-3745
DOI: https://doi.org/10.1007/978-1-62703-791-4_20
Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation Spectroscopy (RICS) are two powerful techniques to study the diffusion dynamics of fluorescently labeled proteins. FRAP and RICS can be easily applied on any commercial confocal microscope. In this chapter, we describe the principles of these methods and provide the reader with a detailed guide on how to apply these methods in the study of Ras nanoclustering and diffusion in the plasma membrane of live cells.
