The aim of the study was to examine age and gender differences in the contents of regrets, and the association between regrets and subjective well-being. The sample consisted of 176 participants ranging in age from 19 to 82 years. The results showed that young adults ( 19 - 29 years) named regrets related to relationships and leisure more often than middle-aged ( 30 - 54 years) and older adults ( 55 - 82 years), whereas regrets related to work and family were more salient among middle-aged and older adults. Furthermore, gender comparison revealed that regrets concerning relationships and family were more frequent among women than among men. Related to subjective well-being, results showed that regrets concerning education and work were negatively associated with life satisfaction, and depressive symptoms associated with self-related regrets.