A1 Refereed original research article in a scientific journal
Human circovirus TT virus genotype 6 expresses six proteins following transfection of a full-length clone
Authors: Qiu J, Kakkola L, Cheng F, Ye C, Söderlund-Venermo M, Hedman K, Pintel DJ
Publication year: 2005
Journal: Journal of Virology
Journal name in source: Journal of virology
Journal acronym: J Virol
Volume: 79
Issue: 10
First page : 6505
Last page: 10
Number of pages: 6
ISSN: 0022-538X
DOI: https://doi.org/10.1128/JVI.79.10.6505-6510.2005
Abstract
The expression profile of the circovirus TTV has not yet been fully characterized. In this paper, we show that following transfection of a full-length viral clone of TTV genotype 6, each of the three virally encoded mRNAs is translated from two initiating AUGs, and therefore, the TTV genome generates at least six proteins. Localization studies of hemagglutinin-tagged versions of these proteins in fixed cells, and green fluorescent protein-tagged versions of these proteins in living cells, expressed following transfection, demonstrated that two were primarily nuclear, two were primarily cytoplasmic, and two were found throughout the cell.
The expression profile of the circovirus TTV has not yet been fully characterized. In this paper, we show that following transfection of a full-length viral clone of TTV genotype 6, each of the three virally encoded mRNAs is translated from two initiating AUGs, and therefore, the TTV genome generates at least six proteins. Localization studies of hemagglutinin-tagged versions of these proteins in fixed cells, and green fluorescent protein-tagged versions of these proteins in living cells, expressed following transfection, demonstrated that two were primarily nuclear, two were primarily cytoplasmic, and two were found throughout the cell.
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