A1 Refereed original research article in a scientific journal
Synthesis of L-2,4-diamino[4-11C]butyric acid and its use in some in vitro and in vivo tumour models
Authors: Antoni G, Omura H, Bergström M, Furuya Y, Moulder R, Roberto A, Sundin A, Watanabe Y, Långström B
Publication year: 1997
Journal: Nuclear Medicine and Biology
Journal name in source: Nuclear medicine and biology
Journal acronym: Nucl Med Biol
Volume: 24
Issue: 6
First page : 595
Last page: 601
Number of pages: 7
ISSN: 0969-8051
DOI: https://doi.org/10.1016/S0969-8051(97)00032-2
Abstract
L-2,4-Diamino[4-11C]butyric acid (DAB) was synthesized by an enzyme catalysed carrier added (0.1 micromol KCN) reaction of hydrogen [11C]cyanide with O-acetyl-L-serine followed by reduction. L-[11C]DAB was obtained with a radiochemical purity higher than 96% and with a decay corrected radiochemical yield of 30-40% within a 32 min reaction time. The enantiomeric excess was 98%. The uptake of L-[11C]DAB was investigated in multicellular aggregates of six different cell lines and animal tumour models. L-[11C]DAB is potentially useful for the assessment of pharmacokinetics of L-DAB in vivo for part of its evaluation as an antitumoural agent, although its use for diagnostic purposes seems limited.
L-2,4-Diamino[4-11C]butyric acid (DAB) was synthesized by an enzyme catalysed carrier added (0.1 micromol KCN) reaction of hydrogen [11C]cyanide with O-acetyl-L-serine followed by reduction. L-[11C]DAB was obtained with a radiochemical purity higher than 96% and with a decay corrected radiochemical yield of 30-40% within a 32 min reaction time. The enantiomeric excess was 98%. The uptake of L-[11C]DAB was investigated in multicellular aggregates of six different cell lines and animal tumour models. L-[11C]DAB is potentially useful for the assessment of pharmacokinetics of L-DAB in vivo for part of its evaluation as an antitumoural agent, although its use for diagnostic purposes seems limited.
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