A1 Refereed original research article in a scientific journal
Sequence and TLR9 independent increase of TRACP expression by antisense DNA and siRNA molecules
Authors: Muhonen P, Avnet S, Parthasarathy RN, Janckila AJ, Halleen JM, Laitala-Leinonen T, Vaananen HK
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Publication year: 2007
Journal: Biochemical and Biophysical Research Communications
Journal name in source: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Journal acronym: BIOCHEM BIOPH RES CO
Volume: 359
Issue: 4
First page : 889
Last page: 895
Number of pages: 7
ISSN: 0006-291X
DOI: https://doi.org/10.1016/j.bbrc.2007.05.205
Abstract
Reactive oxygen species generating activity of tart rate-resistant acid phosphatase (TRACP) has been suggested to have several functions in TRACP expressing bone resorbing osteoclasts, macrophages, and dendritic cells. This work aimed to study the TRACP knock down phenotype in osteoclasts by using antisense DNA and RNA interference methods. Unexpectedly, both TRACP specific DNA oligonucteotides and siRNA molecules extensively increased the TRACP expression in human osteoclasts and monocytes. Toll-like receptor 9 (TLR9) is an immunity sensor for CpG motifs in DNA. We cultured bone marrow-derived osteoclast precursor cells from wild-type and TLR9-/- mice with CpG and non-CpG DNA oligonucleotides, and observed that the increased TRACP expression was sequence and TLR9 independent. In contrast, cells with increased TRACP activity showed decreased activity of tartrate-sensitive acid phosphatases. Conclusion: DNA oligonucleotides and RNA molecules extensively increase TRACP expression in monocyte-macrophage lineage. These results suggest a potential role of TRACP in pathogen recognition and in innate immunity. (c) 2007 Elsevier Inc. All rights reserved.
Reactive oxygen species generating activity of tart rate-resistant acid phosphatase (TRACP) has been suggested to have several functions in TRACP expressing bone resorbing osteoclasts, macrophages, and dendritic cells. This work aimed to study the TRACP knock down phenotype in osteoclasts by using antisense DNA and RNA interference methods. Unexpectedly, both TRACP specific DNA oligonucteotides and siRNA molecules extensively increased the TRACP expression in human osteoclasts and monocytes. Toll-like receptor 9 (TLR9) is an immunity sensor for CpG motifs in DNA. We cultured bone marrow-derived osteoclast precursor cells from wild-type and TLR9-/- mice with CpG and non-CpG DNA oligonucleotides, and observed that the increased TRACP expression was sequence and TLR9 independent. In contrast, cells with increased TRACP activity showed decreased activity of tartrate-sensitive acid phosphatases. Conclusion: DNA oligonucleotides and RNA molecules extensively increase TRACP expression in monocyte-macrophage lineage. These results suggest a potential role of TRACP in pathogen recognition and in innate immunity. (c) 2007 Elsevier Inc. All rights reserved.
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