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PREFERENTIAL USAGE OF T-CELL ANTIGEN RECEPTOR-V REGION GENE SEGMENT V-BETA-5.1 BY BORRELIA-BURGDORFERI ANTIGEN-REACTIVE T-CELL CLONES ISOLATED FROM A PATIENT WITH LYME-DISEASE
Tekijät: LAHESMAA R, SHANAFELT MC, ALLSUP A, SODERBERG C, ANZOLA J, FREITAS V, TURCK C, STEINMAN L, PELTZ G
Kustantaja: AMER ASSOC IMMUNOLOGISTS
Julkaisuvuosi: 1993
Journal: Journal of Immunology
Tietokannassa oleva lehden nimi: JOURNAL OF IMMUNOLOGY
Lehden akronyymi: J IMMUNOL
Vuosikerta: 150
Numero: 9
Aloitussivu: 4125
Lopetussivu: 4135
Sivujen määrä: 11
ISSN: 0022-1767
Tiivistelmä
Forty-three CD3+4+8- TCRalphabeta+ Borrelia burgdorferi-reactive T cell clones isolated from the peripheral blood of a single patient with clinically active chronic Lyme arthritis were characterized. The spirochetal Ag recognized by 16 of these T cell clones was determined by reactivity with a panel of recombinant spirochetal Ag, which included the OspA, OspB, flagellin, Hsp60 and Hsp70 proteins. All three T cell clones reactive with heat shock proteins recognized a non-cross-reactive epitope unique to the spirochetal Ag. Analysis of the TCR V regions revealed preferential usage of Vbeta5.1; 5 of 15 T cell clones that recognized an unidentified spirochetal Ag utilized this Vbeta gene segment. Most of the T cell clones recognized a given spirochetal Ag exclusively within the context of one HLA class II allele. However, two T cell clones, which recognized an unidentified Ag in the spirochetal lysate within the context of different HLA class II alleles, were both TCR Vbeta5.1+, although each displayed a distinct alpha-chain. Moreover, in vitro incubation of this patient's PBMC with B. burgdorferi Ag resulted in a specific increase in the percentage of T cells expressing TCR Vbeta5.1. These results indicate that B. burgdorferi has a Vbeta-selective factor influencing the cellular immune response in a patient with clinically active Lyme disease.
Forty-three CD3+4+8- TCRalphabeta+ Borrelia burgdorferi-reactive T cell clones isolated from the peripheral blood of a single patient with clinically active chronic Lyme arthritis were characterized. The spirochetal Ag recognized by 16 of these T cell clones was determined by reactivity with a panel of recombinant spirochetal Ag, which included the OspA, OspB, flagellin, Hsp60 and Hsp70 proteins. All three T cell clones reactive with heat shock proteins recognized a non-cross-reactive epitope unique to the spirochetal Ag. Analysis of the TCR V regions revealed preferential usage of Vbeta5.1; 5 of 15 T cell clones that recognized an unidentified spirochetal Ag utilized this Vbeta gene segment. Most of the T cell clones recognized a given spirochetal Ag exclusively within the context of one HLA class II allele. However, two T cell clones, which recognized an unidentified Ag in the spirochetal lysate within the context of different HLA class II alleles, were both TCR Vbeta5.1+, although each displayed a distinct alpha-chain. Moreover, in vitro incubation of this patient's PBMC with B. burgdorferi Ag resulted in a specific increase in the percentage of T cells expressing TCR Vbeta5.1. These results indicate that B. burgdorferi has a Vbeta-selective factor influencing the cellular immune response in a patient with clinically active Lyme disease.
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