A1 Refereed original research article in a scientific journal

APPLICATION OF THE POLYMERASE CHAIN-REACTION AND IMMUNOFLUORESCENCE TECHNIQUES TO THE DETECTION OF BACTERIA IN YERSINIA-TRIGGERED REACTIVE ARTHRITIS




AuthorsVIITANEN AM, ARSTILA TP, LAHESMAA R, GRANFORS K, SKURNIK M, TOIVANEN P

PublisherLIPPINCOTT-RAVEN PUBL

Publication year1991

JournalArthritis and Rheumatism

Journal name in sourceARTHRITIS AND RHEUMATISM

Journal acronymARTHRITIS RHEUM

Volume34

Issue1

First page 89

Last page96

Number of pages8

ISSN0004-3591

DOIhttps://doi.org/10.1002/art.1780340114(external)


Abstract
Leukocytes in synovial fluid and peripheral blood samples from patients with Yersinia-triggered reactive arthritis were analyzed after DNA amplification using the polymerase chain reaction. The primers applied were specific for the virulence plasmid-coded 1crE genes of Yersinia enterocolitica O:3 and Yersinia pseudotuberculosis III. No Yersinia DNA was observed within the synovial fluid cells or peripheral blood cells by polymerase chain reaction techniques. However, Yersinia antigens were detected in the synovial fluid cells by immunofluorescence techniques. These results suggest that only parts of the causative agents, not the entire microbe, can enter the joint and initiate the inflammation that leads to a reactive arthritis.



Last updated on 2024-26-11 at 22:42