A1 Refereed original research article in a scientific journal

RIG-I Signaling Is Essential for Influenza B Virus-Induced Rapid Interferon Gene Expression




AuthorsSanna M. Mäkelä, Pamela Österlund, Veera Westenius, Sinikka Latvala, Michael S. Diamond, Michael Gale Jr., Ilkka Julkunen

PublisherAMER SOC MICROBIOLOGY

Publication year2015

JournalJournal of Virology

Journal name in sourceJOURNAL OF VIROLOGY

Journal acronymJ VIROL

Volume89

Issue23

First page 12014

Last page12025

Number of pages12

ISSN0022-538X

eISSN1098-5514

DOIhttps://doi.org/10.1128/JVI.01576-15(external)


Abstract

Recently, a great deal of interest has been paid to identifying the ligands for RIG-I under conditions of natural infection, as many previous studies have been based on transfection of cells with different types of viral or synthetic RNA structures. We shed light on this question by analyzing the earliest step in innate immune recognition of influenza B virus by human macrophages. We show that influenza B virus induces IRF3 activation, leading to IFN gene expression after viral RNPs (vRNPs) are released into the cytosol and are recognized by RIG-I receptor, meaning that the incoming influenza B virus is already able to activate IFN gene expression. In contrast, influenza A (H3N2) virus failed to activate IRF3 at very early times of infection, suggesting that there are differences in innate immune recognition between influenza A and B viruses.




Last updated on 2024-26-11 at 18:37