A1 Refereed original research article in a scientific journal
YERSINIA-SPECIFIC ANTIBODIES IN SERUM AND SYNOVIAL-FLUID IN PATIENTS WITH YERSINIA TRIGGERED REACTIVE ARTHRITIS
Authors: MAKIIKOLA O, LAHESMAA R, HEESEMANN J, MERILAHTIPALO R, SAARIO R, TOIVANEN A, GRANFORS K
Publisher: BRITISH MED JOURNAL PUBL GROUP
Publication year: 1994
Journal: Annals of the Rheumatic Diseases
Journal name in source: ANNALS OF THE RHEUMATIC DISEASES
Journal acronym: ANN RHEUM DIS
Volume: 53
Issue: 8
First page : 535
Last page: 539
Number of pages: 5
ISSN: 0003-4967
DOI: https://doi.org/10.1136/ard.53.8.535
Abstract
Objectives-To further evaluate the role of bacterial antigens in triggering inflammation in patients with by studying local antibody synthesis in the joint.Methods-Yersinia-specific antibodies in paired serum and synovial fluid samples from 29 patients with yersinia triggered reactive arthritis were studied using an enzyme linked immunosorbent assay (ELISA), an inhibition ELISA with six monoclonal antibodies against Lipopolysaccharide or released proteins of yersinia and immunoblotting. Antibodies of IgM, IgG and IgA classes, as well as antibodies of IgA subclasses and those containing secretory component were measured against the lipopolysaccharide and the sodium dodecyl sulphate extract of whole Yersinia enterocolitica O:3 bacteria.Results-It was shown that yersinia-specific antibodies, as well as antibodies against other microbial antigens (rubella, measles, Bordetella pertussis, tetanus toroid and Candida albicans) in synovial fluid mirror those in serum by concentration, by specificity and by distribution in classes and subclasses.Conclusion-These results do not suggest any strong local antibody production, but indicate that the majority of yersinia antibodies in the synovial fluid are derived from the circulation.
Objectives-To further evaluate the role of bacterial antigens in triggering inflammation in patients with by studying local antibody synthesis in the joint.Methods-Yersinia-specific antibodies in paired serum and synovial fluid samples from 29 patients with yersinia triggered reactive arthritis were studied using an enzyme linked immunosorbent assay (ELISA), an inhibition ELISA with six monoclonal antibodies against Lipopolysaccharide or released proteins of yersinia and immunoblotting. Antibodies of IgM, IgG and IgA classes, as well as antibodies of IgA subclasses and those containing secretory component were measured against the lipopolysaccharide and the sodium dodecyl sulphate extract of whole Yersinia enterocolitica O:3 bacteria.Results-It was shown that yersinia-specific antibodies, as well as antibodies against other microbial antigens (rubella, measles, Bordetella pertussis, tetanus toroid and Candida albicans) in synovial fluid mirror those in serum by concentration, by specificity and by distribution in classes and subclasses.Conclusion-These results do not suggest any strong local antibody production, but indicate that the majority of yersinia antibodies in the synovial fluid are derived from the circulation.
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