A1 Refereed original research article in a scientific journal
CD45 isoforms on human CD4(+) T-cell subsets
Authors: Shanafelt MC, Yssel H, Soderberg C, Steinman L, Adelman DC, Peltz G, Lahesmaa R
Publisher: MOSBY-YEAR BOOK INC
Publication year: 1996
Journal: Journal of Allergy and Clinical Immunology
Journal name in source: JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
Journal acronym: J ALLERGY CLIN IMMUN
Volume: 98
Issue: 2
First page : 433
Last page: 440
Number of pages: 8
ISSN: 0091-6749
DOI: https://doi.org/10.1016/S0091-6749(96)70168-9
Abstract
We have examined the level of surface expression and functional properties of leukocyte function associated antigen-1, very late antigen-4, and CD45 isoforms on a panel of human CD4(+) T-cell clones representative of TH0, TH1, and TH2 cells. There were no qualitative differences in the expression of these antigens among the three types of CD4(+) T-cell clones. However, CD45RB was the only CD45 isoform that provided a costimulatory signal in a solid-phase antibody-induced cellular proliferation assay. Additionally, the antigen-induced proliferative response of T-cell clones was inhibited by soluble anti-CD45RO and anti-CD45RB antibodies. Our results suggest that CD45 informs differentially provide costimulatory signals to T cells. However, the ability of each CD45 isoforms to provide a costimulatory signal does not differ among the Th-0, TH1, or TH2 T-cell populations.
We have examined the level of surface expression and functional properties of leukocyte function associated antigen-1, very late antigen-4, and CD45 isoforms on a panel of human CD4(+) T-cell clones representative of TH0, TH1, and TH2 cells. There were no qualitative differences in the expression of these antigens among the three types of CD4(+) T-cell clones. However, CD45RB was the only CD45 isoform that provided a costimulatory signal in a solid-phase antibody-induced cellular proliferation assay. Additionally, the antigen-induced proliferative response of T-cell clones was inhibited by soluble anti-CD45RO and anti-CD45RB antibodies. Our results suggest that CD45 informs differentially provide costimulatory signals to T cells. However, the ability of each CD45 isoforms to provide a costimulatory signal does not differ among the Th-0, TH1, or TH2 T-cell populations.
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