A1 Refereed original research article in a scientific journal
Osteoblast recruitment from stem cells does not decrease by age at late adulthood
Authors: Leskela HV, Risteli J, Niskanen S, Koivunen J, Ivaska KK, Lehenkari P
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Publication year: 2003
Journal: Biochemical and Biophysical Research Communications
Journal name in source: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Journal acronym: BIOCHEM BIOPH RES CO
Volume: 311
Issue: 4
First page : 1008
Last page: 1013
Number of pages: 6
ISSN: 0006-291X
DOI: https://doi.org/10.1016/j.bbrc.2003.10.095
Abstract
This study was aimed to characterize the ability of human bone marrow mesenchymal stem cells (MSC) to differentiate into osteoblasts in vitro. Twenty-three women and 20 men at late adulthood (52-92 years of age) were selected for the study. MSCs were isolated and cultured in vitro and alkaline phosphatase (ALP) activity, secretion of amino-terminal propeptide of type I procollagen (PINP), type III procollagen (PIIINP) and osteocalcin were analyzed. Matrix mineralization was analyzed by the von Kossa staining and by calcium quantification. We found that the ALP and PINP levels compared with control increased to 2.8- and 2.9-fold, respectively, when cells were cultured for three weeks. ALP activity, PINP and calcium deposition in response to dexamethasone treatment increased by age in women and was unchanged in men. Overall our data suggests that the osteogenic potential of MSCs does not decrease by age in either women or men at late adulthood. (C) 2003 Elsevier Inc. All rights reserved.
This study was aimed to characterize the ability of human bone marrow mesenchymal stem cells (MSC) to differentiate into osteoblasts in vitro. Twenty-three women and 20 men at late adulthood (52-92 years of age) were selected for the study. MSCs were isolated and cultured in vitro and alkaline phosphatase (ALP) activity, secretion of amino-terminal propeptide of type I procollagen (PINP), type III procollagen (PIIINP) and osteocalcin were analyzed. Matrix mineralization was analyzed by the von Kossa staining and by calcium quantification. We found that the ALP and PINP levels compared with control increased to 2.8- and 2.9-fold, respectively, when cells were cultured for three weeks. ALP activity, PINP and calcium deposition in response to dexamethasone treatment increased by age in women and was unchanged in men. Overall our data suggests that the osteogenic potential of MSCs does not decrease by age in either women or men at late adulthood. (C) 2003 Elsevier Inc. All rights reserved.
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