A1 Refereed original research article in a scientific journal
Identification of novel proteolytic forms of osteocalcin in human urine
Authors: Ivaska KK, Hellman J, Likojarvi J, Kakonen SM, Gerdhem P, Akesson K, Obrant KJ, Pettersson K, Vaananen HK
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Publication year: 2003
Journal: Biochemical and Biophysical Research Communications
Journal name in source: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Journal acronym: BIOCHEM BIOPH RES CO
Volume: 306
Issue: 4
First page : 973
Last page: 980
Number of pages: 8
ISSN: 0006-291X
DOI: https://doi.org/10.1016/S0006-291X(03)01093-3
Abstract
In this study, we report the isolation and characterization of osteocalcin in human urine using mass spectrometry and N-terminal sequencing. Multiple proteolytic forms of osteocalcin were found, which consisted of 16-27 residues from the middle region of the molecule. Several fragments had residue Gly7 at the N-terminus and the most predominant was fragment 7-31. Additional fragments starting from residue Asp14 were detected in the samples of children and young adults. Immunochemical detection of urine osteocalcin fragments had a statistically significant negative correlation to bone mineral density in evaluation of urine samples from 75-year-old women. Thus, the measurement of osteocalcin fragments in urine may have potential applications in diagnostics related to disorders of bone metabolism. (C) 2003 Elsevier Science (USA). All rights reserved.
In this study, we report the isolation and characterization of osteocalcin in human urine using mass spectrometry and N-terminal sequencing. Multiple proteolytic forms of osteocalcin were found, which consisted of 16-27 residues from the middle region of the molecule. Several fragments had residue Gly7 at the N-terminus and the most predominant was fragment 7-31. Additional fragments starting from residue Asp14 were detected in the samples of children and young adults. Immunochemical detection of urine osteocalcin fragments had a statistically significant negative correlation to bone mineral density in evaluation of urine samples from 75-year-old women. Thus, the measurement of osteocalcin fragments in urine may have potential applications in diagnostics related to disorders of bone metabolism. (C) 2003 Elsevier Science (USA). All rights reserved.
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