A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Analysis of Indole Alkaloids from Rhazya stricta Hairy Roots by Ultra-Performance Liquid Chromatography-Mass Spectrometry
Tekijät: Amir Akhgari, Into Laakso, Tuulikki Seppänen-Laakso, Teijo Yrjönen, Heikki Vuorela, Kirsi-Marja Oksman-Caldentey, Heiko Rischer
Julkaisuvuosi: 2015
Lehti:: Molecules
Vuosikerta: 20
Numero: 12
Aloitussivu: 22621
Lopetussivu: 22634
Tiivistelmä
Rhazya stricta Decne. (Apocynaceae) contains a large number of terpenoid indolealkaloids (TIAs). This study focused on the composition of alkaloids obtained from transformedhairy root cultures of R. stricta employing ultra-performance liquid chromatography-massspectrometry (UPLC-MS). In the UPLC-MS analyses, a total of 20 TIAs were identified from crudeextracts. Eburenine and vincanine were the main alkaloids followed by polar glucoalkaloids,strictosidine lactam and strictosidine. Secodine-type alkaloids, tetrahydrosecodinol, tetrahydro- anddihydrosecodine were detected too. The occurrence of tetrahydrosecodinol was confirmed for thefirst time for R. stricta. Furthermore, two isomers of yohimbine, serpentine and vallesiachotaminewere identified. The study shows that a characteristic pattern of biosynthetically related TIAs canbe monitored in Rhazya hairy root crude extract by this chromatographic method.
Rhazya stricta Decne. (Apocynaceae) contains a large number of terpenoid indolealkaloids (TIAs). This study focused on the composition of alkaloids obtained from transformedhairy root cultures of R. stricta employing ultra-performance liquid chromatography-massspectrometry (UPLC-MS). In the UPLC-MS analyses, a total of 20 TIAs were identified from crudeextracts. Eburenine and vincanine were the main alkaloids followed by polar glucoalkaloids,strictosidine lactam and strictosidine. Secodine-type alkaloids, tetrahydrosecodinol, tetrahydro- anddihydrosecodine were detected too. The occurrence of tetrahydrosecodinol was confirmed for thefirst time for R. stricta. Furthermore, two isomers of yohimbine, serpentine and vallesiachotaminewere identified. The study shows that a characteristic pattern of biosynthetically related TIAs canbe monitored in Rhazya hairy root crude extract by this chromatographic method.
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