A1 Refereed original research article in a scientific journal
Evaluation of a commercial MRSA assay when multiple MRSA strains are causing epidemics
Authors: Sissonen S, Pasanen T, Salmenlinna S, Vuopio-Varkila J, Tarkka E, Vaara M, Tissari P
Publication year: 2009
Journal: European Journal of Clinical Microbiology and Infectious Diseases
Journal name in source: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Journal acronym: Eur J Clin Microbiol Infect Dis
Volume: 28
Issue: 10
First page : 1271
Last page: 3
Number of pages: 3
ISSN: 0934-9723
eISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-009-0771-z
Abstract
Rapid and reliable diagnostic methods are needed to control methicillin-resistant Staphylococcus aureus (MRSA) transmission. We studied the BD GeneOhm MRSA Assay which is based on one specific amplification product at the junction of the right extremity sequence of the staphylococcal cassette chromosome mec (SCCmec) and the chromosomal sequence of orfX of S. aureus. The test was applied on 95 clinical isolates in Finland: 83% were positive. The isolates giving negative results represented several pulsed-field gel electrophoresis (PFGE) types and harboured SCCmec types IV, V, VI or were new types with different combinations of ccr genes.
Rapid and reliable diagnostic methods are needed to control methicillin-resistant Staphylococcus aureus (MRSA) transmission. We studied the BD GeneOhm MRSA Assay which is based on one specific amplification product at the junction of the right extremity sequence of the staphylococcal cassette chromosome mec (SCCmec) and the chromosomal sequence of orfX of S. aureus. The test was applied on 95 clinical isolates in Finland: 83% were positive. The isolates giving negative results represented several pulsed-field gel electrophoresis (PFGE) types and harboured SCCmec types IV, V, VI or were new types with different combinations of ccr genes.
UTU Research Portal