A1 Refereed original research article in a scientific journal
A proteome database of human primary T helper cells
Authors: Nyman TA, Rosengren A, Syyrakki S, Pellinen TP, Rautajoki K, Lahesmaa R
Publisher: WILEY-V C H VERLAG GMBH
Publication year: 2001
Journal: ELECTROPHORESIS
Journal name in source: ELECTROPHORESIS
Journal acronym: ELECTROPHORESIS
Volume: 22
Issue: 20
First page : 4375
Last page: 4382
Number of pages: 8
ISSN: 0173-0835
DOI: https://doi.org/10.1002/1522-2683(200112)22:20<4375::AID-ELPS4375>3.0.CO;2-P
Abstract
We have established the first public database of human primary T helper cell proteome using two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight-mass spectrometry. For the database, CD4(+) human T cells were activated with anti-CD3+anti-CD28 antibodies and metabolically labeled with [S-35]methionine for 24 h. Cells were lysed and proteins were separated by 2-DE. About 1500 protein spots were detected in the resulting 2-DE gels with silver staining, and 2000 spots with autoradiography. We have identified 91 proteins from the 2-DE gels using peptide mass fingerprinting, and annotated them to our database. The identified proteins are also linked to SWISS-PROT and NCBI protein databases. Our database is available via the Internet at http://www3.btk.utu.fi:8080/Genomics/Proteomics/Database.
We have established the first public database of human primary T helper cell proteome using two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight-mass spectrometry. For the database, CD4(+) human T cells were activated with anti-CD3+anti-CD28 antibodies and metabolically labeled with [S-35]methionine for 24 h. Cells were lysed and proteins were separated by 2-DE. About 1500 protein spots were detected in the resulting 2-DE gels with silver staining, and 2000 spots with autoradiography. We have identified 91 proteins from the 2-DE gels using peptide mass fingerprinting, and annotated them to our database. The identified proteins are also linked to SWISS-PROT and NCBI protein databases. Our database is available via the Internet at http://www3.btk.utu.fi:8080/Genomics/Proteomics/Database.
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