A1 Refereed original research article in a scientific journal
Strategy for Nuclear-Magnetic-Resonance-Based Metabolomics of Human Feces
Authors: Lamichhane S, Yde CC, Schmedes MS, Jensen HM, Meier S, Bertram HC
Publisher: AMER CHEMICAL SOC
Publication year: 2015
Journal: Analytical Chemistry
Journal name in source: ANALYTICAL CHEMISTRY
Journal acronym: ANAL CHEM
Volume: 87
Issue: 12
First page : 5930
Last page: 5937
Number of pages: 8
ISSN: 0003-2700
DOI: https://doi.org/10.1021/acs.analchem.5b00977
Abstract
Metabolomic analyses of fecal material are gaining increasing attention because the gut microbial ecology and activity have an impact on the human phenotype and regulate host metabolism. :Sample preparation is a crucial step, and in this study, we recommend a methodology for extraction and analysis of fresh feces by NMR-based metabolomics. The evaluation of extraction solvents showed that buffet extraction is a suitable approach to extract metabolic information in feces. Therefore, the effects of weight-to-buffer (Wf:Vb) combinations and the effect of sonication and freeze thaw cycles on the reproducibility, chemical shift variability, and signal-to-noise ratio (SNR) of the H-1 NMR spectra were evaluated: On the basis of our results, we suggest that fresh fecal extraction with a Wf:Vb ratio of 1:2 may be the optimum choice to determine the overall metabolite composition of feces. In fact, more than 60 metabolites have been assigned in the NMR spectra obtained from the fresh fecal buffer extract, and assignments of the lipophilic signals are also presented. TO our knowledge, some of the metabolites are reported here for the very first time employing H-1 NMR spectroscopy on human fecal extracts.
Metabolomic analyses of fecal material are gaining increasing attention because the gut microbial ecology and activity have an impact on the human phenotype and regulate host metabolism. :Sample preparation is a crucial step, and in this study, we recommend a methodology for extraction and analysis of fresh feces by NMR-based metabolomics. The evaluation of extraction solvents showed that buffet extraction is a suitable approach to extract metabolic information in feces. Therefore, the effects of weight-to-buffer (Wf:Vb) combinations and the effect of sonication and freeze thaw cycles on the reproducibility, chemical shift variability, and signal-to-noise ratio (SNR) of the H-1 NMR spectra were evaluated: On the basis of our results, we suggest that fresh fecal extraction with a Wf:Vb ratio of 1:2 may be the optimum choice to determine the overall metabolite composition of feces. In fact, more than 60 metabolites have been assigned in the NMR spectra obtained from the fresh fecal buffer extract, and assignments of the lipophilic signals are also presented. TO our knowledge, some of the metabolites are reported here for the very first time employing H-1 NMR spectroscopy on human fecal extracts.
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