A1 Refereed original research article in a scientific journal
Optimizing sampling strategies for NMR-based metabolomics of human feces: pooled vs. unpooled analyses
Authors: Lamichhane S, Sundekilde UK, Blaedel T, Dalsgaard TK, Larsen LH, Dragsted LO, Astrup A, Bertram HC
Publisher: ROYAL SOC CHEMISTRY
Publication year: 2017
Journal: Analytical Methods
Journal name in source: ANALYTICAL METHODS
Journal acronym: ANAL METHODS-UK
Volume: 9
Issue: 30
First page : 4476
Last page: 4480
Number of pages: 5
ISSN: 1759-9660
DOI: https://doi.org/10.1039/c7ay01465a
Abstract
This study focused on elucidating fecal sample collection for 1H NMR-based metabolite profiling. Pooled vs. unpooled feces sampling was compared to determine if single unpooled samples are adequate and representative for NMR based metabolomics of feces. Our results show that the metabolome of unpooled fecal samples varied within the individual (day-to-day variation). This variation could mainly be ascribed to fluctuations (CV > 40%) in the level of the metabolites glucose, lactate, and succinate. Consequently, our data suggest that multiple sampling and pooling of fecal samples could be considered to minimize errors arising from day to day variation in fecal sample.
This study focused on elucidating fecal sample collection for 1H NMR-based metabolite profiling. Pooled vs. unpooled feces sampling was compared to determine if single unpooled samples are adequate and representative for NMR based metabolomics of feces. Our results show that the metabolome of unpooled fecal samples varied within the individual (day-to-day variation). This variation could mainly be ascribed to fluctuations (CV > 40%) in the level of the metabolites glucose, lactate, and succinate. Consequently, our data suggest that multiple sampling and pooling of fecal samples could be considered to minimize errors arising from day to day variation in fecal sample.
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