A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Proteome profiling of interleukin-12 treated human T helper cells
Tekijät: Rosengren AT, Nyman TA, Lahesmaa R
Kustantaja: WILEY-V C H VERLAG GMBH
Julkaisuvuosi: 2005
Journal: Proteomics
Tietokannassa oleva lehden nimi: PROTEOMICS
Lehden akronyymi: PROTEOMICS
Vuosikerta: 5
Numero: 12
Aloitussivu: 3137
Lopetussivu: 3141
Sivujen määrä: 5
ISSN: 1615-9853
DOI: https://doi.org/10.1002/pmic.200401151
Tiivistelmä
Selective activation of T helper subsets 1 (Th1) and 2 (M) plays a crucial role in different pathological conditions. Th1 cell response is involved in pathogenesis of autoimmune diseases, such as type 11 diabetes and multiple sclerosis, and Th2 cell response in pathogenesis of allergy and asthma. Cytokine interleukin-12 (IL-12) is one of the key factors in the differentiation of naive CD4(+) T cells into Th1 cells. In this study we used 2-DE and MS to find and identify IL-12 regulated proteins in human CD4(+) T cells. In total, 42 protein spots were found to be differentially expressed following IL-12 stimulation, of which 22 were up- and 20 down-regulated. Among the upregulated proteins there are a multifunctional cytokine macrophage migration inhibitory factor and a known IL-12 target gene Programmed cell death 4. Downregulated proteins include p21-activated kinase 2 and its upstream GTPase Cdc42. Compared to previous reports our analysis provides a new view on the IL-12 induced changes on CD4(+) Tcells underscoring the importance of creating and combining the data generated at various levels to build a comprehensive view of a given biological process of the cell.
Selective activation of T helper subsets 1 (Th1) and 2 (M) plays a crucial role in different pathological conditions. Th1 cell response is involved in pathogenesis of autoimmune diseases, such as type 11 diabetes and multiple sclerosis, and Th2 cell response in pathogenesis of allergy and asthma. Cytokine interleukin-12 (IL-12) is one of the key factors in the differentiation of naive CD4(+) T cells into Th1 cells. In this study we used 2-DE and MS to find and identify IL-12 regulated proteins in human CD4(+) T cells. In total, 42 protein spots were found to be differentially expressed following IL-12 stimulation, of which 22 were up- and 20 down-regulated. Among the upregulated proteins there are a multifunctional cytokine macrophage migration inhibitory factor and a known IL-12 target gene Programmed cell death 4. Downregulated proteins include p21-activated kinase 2 and its upstream GTPase Cdc42. Compared to previous reports our analysis provides a new view on the IL-12 induced changes on CD4(+) Tcells underscoring the importance of creating and combining the data generated at various levels to build a comprehensive view of a given biological process of the cell.
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