A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Gene expression profiling during differentiation of human monocytes to macrophages or dendritic cells
Tekijät: Lehtonen A, Ahlfors H, Veckman V, Miettinen M, Lahesmaa R, Julkunen I
Kustantaja: FEDERATION AMER SOC EXP BIOL
Julkaisuvuosi: 2007
Journal: Journal of Leukocyte Biology
Tietokannassa oleva lehden nimi: JOURNAL OF LEUKOCYTE BIOLOGY
Lehden akronyymi: J LEUKOCYTE BIOL
Vuosikerta: 82
Numero: 3
Aloitussivu: 710
Lopetussivu: 720
Sivujen määrä: 11
ISSN: 0741-5400
DOI: https://doi.org/10.1189/jlb.0307194
Tiivistelmä
Macrophages and dendritic cells (DC) are APC, which regulate innate and adaptive immune responses. Macrophages function locally mainly, maintaining inflammatory responses in tissues, whereas DC take up microbes, mature, and migrate to local lymph nodes to present microbial antigens to naive T cells to elicit microbe-specific immune responses. Blood monocytes can be differentiated in vitro to macrophages or DC by GM-CSF or GM-CSF + IL-4, respectively. In the present study, we performed global gene expression analyses using Affymetrix HG-U133A Gene Chip oligonucleotide arrays during macrophage and DC differentiation. During the differentiation process, 340 and 350 genes were up-regulated, and 190 and 240 genes were down-regulated in macrophages and DC, respectively. There were also more that 200 genes, which were expressed differentially in fully differentiated macrophages and DC. Macrophage-specific genes include, e. g., CD14, CD163, C5R1, and Fc gamma R1A, and several cell surface adhesion molecules, cytokine receptors, WNT5A and its receptor of the Frizzled family FZD2, fibronectin, and Fc gamma R1A were identified as DC-specific. Our results reveal significant differences in gene expression profiles between macrophages and DC, and these differences can partially explain the functional differences between these two important cell types.
Macrophages and dendritic cells (DC) are APC, which regulate innate and adaptive immune responses. Macrophages function locally mainly, maintaining inflammatory responses in tissues, whereas DC take up microbes, mature, and migrate to local lymph nodes to present microbial antigens to naive T cells to elicit microbe-specific immune responses. Blood monocytes can be differentiated in vitro to macrophages or DC by GM-CSF or GM-CSF + IL-4, respectively. In the present study, we performed global gene expression analyses using Affymetrix HG-U133A Gene Chip oligonucleotide arrays during macrophage and DC differentiation. During the differentiation process, 340 and 350 genes were up-regulated, and 190 and 240 genes were down-regulated in macrophages and DC, respectively. There were also more that 200 genes, which were expressed differentially in fully differentiated macrophages and DC. Macrophage-specific genes include, e. g., CD14, CD163, C5R1, and Fc gamma R1A, and several cell surface adhesion molecules, cytokine receptors, WNT5A and its receptor of the Frizzled family FZD2, fibronectin, and Fc gamma R1A were identified as DC-specific. Our results reveal significant differences in gene expression profiles between macrophages and DC, and these differences can partially explain the functional differences between these two important cell types.
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