A1 Refereed original research article in a scientific journal
Distinct sets of developmentally regulated genes that are expressed by human oocytes and human embryonic stem cells
Authors: Zhang P, Kerkelä E, Skottman H, Levkov L, Kivinen K, Lahesmaa R, Hovatta O, Kere J
Publication year: 2007
Journal: Fertility and Sterility
Journal name in source: Fertility and sterility
Journal acronym: Fertil Steril
Volume: 87
Issue: 3
First page : 677
Last page: 90
Number of pages: 14
ISSN: 0015-0282
eISSN: 1556-5653
DOI: https://doi.org/10.1016/j.fertnstert.2006.07.1509
Abstract
We obtained, for the first time, a large amount of information on gene expression of hGVO as compared with hESC. These data, from a unique research material-human oocytes, can now be used to understand the molecular mechanisms of early human development.\nTo identify genes that are expressed differently during final oocyte maturation and early embryonic development in humans.\nComparison of gene expression profiles of human germinal vesicle oocytes (hGVO), human embryonic stem cells (hESC) and human foreskin fibroblasts.\nResearch centers and a fertility unit in a university hospital.\nFifty-five healthy women donated 76 hGVO.\nNone.\nGene expression profiles were analyzed and compared with the use of microarray and reverse-transcription polymerase chain reaction.\nAltogether, 10,183 genes were expressed in hGVO, and 45% of these genes were unclassified by biologic function. Four oocyte-specific genes (MATER, ZAR1, NPM2 and FIGLA) were detected in hGVO for the first time. Known components of 4 signaling pathways (MOS-MPF, transforming growth factor-beta, WNT, and NOTCH) were also found expressed in hGVO, with some components detected in hGVO for the first time. Distinct sets of genes that were revealed by comparison of expression profiles between hGVO, hESC, and human foreskin fibroblasts appear to be involved in oocyte maturation and early embryonic development.\nCONCLUSION(S)\nOBJECTIVE\nDESIGN\nSETTING\nPATIENT(S)\nINTERVENTION(S)\nMAIN OUTCOME MEASURE(S)\nRESULT(S)
We obtained, for the first time, a large amount of information on gene expression of hGVO as compared with hESC. These data, from a unique research material-human oocytes, can now be used to understand the molecular mechanisms of early human development.\nTo identify genes that are expressed differently during final oocyte maturation and early embryonic development in humans.\nComparison of gene expression profiles of human germinal vesicle oocytes (hGVO), human embryonic stem cells (hESC) and human foreskin fibroblasts.\nResearch centers and a fertility unit in a university hospital.\nFifty-five healthy women donated 76 hGVO.\nNone.\nGene expression profiles were analyzed and compared with the use of microarray and reverse-transcription polymerase chain reaction.\nAltogether, 10,183 genes were expressed in hGVO, and 45% of these genes were unclassified by biologic function. Four oocyte-specific genes (MATER, ZAR1, NPM2 and FIGLA) were detected in hGVO for the first time. Known components of 4 signaling pathways (MOS-MPF, transforming growth factor-beta, WNT, and NOTCH) were also found expressed in hGVO, with some components detected in hGVO for the first time. Distinct sets of genes that were revealed by comparison of expression profiles between hGVO, hESC, and human foreskin fibroblasts appear to be involved in oocyte maturation and early embryonic development.\nCONCLUSION(S)\nOBJECTIVE\nDESIGN\nSETTING\nPATIENT(S)\nINTERVENTION(S)\nMAIN OUTCOME MEASURE(S)\nRESULT(S)
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