A1 Refereed original research article in a scientific journal
Free secretory component as a standardization protein for nasopharyngeal specimens from children with upper respiratory tract infection
Authors: Heikkinen T, Shenoy M, Goldblum RM, Chonmaitree T
Publisher: SCANDINAVIAN UNIVERSITY PRESS
Publication year: 1999
Journal: Acta Paediatrica
Journal name in source: ACTA PAEDIATRICA
Journal acronym: ACTA PAEDIATR
Volume: 88
Issue: 2
First page : 150
Last page: 153
Number of pages: 4
ISSN: 0803-5253
DOI: https://doi.org/10.1080/08035259950170303(external)
Abstract
Free secretory component (FSC) has been recommended as a reliable protein for correction of the unknown dilution in tracheal aspirate samples from preterm infants. To investigate whether FSC would also provide a valid standardization protein for samples of nasopharyngeal secretions, this study determined the intersubject variation and the alteration over time in the concentrations of FSC in nasal secretions from 35 children (median age 14 months) who participated in an antibiotic efficacy trial. Nasopharyngeal aspirates were obtained at enrolment and after 2-3 d. FSC in the specimens was quantified by a direct enzyme immunoassay. The concentrations of FSC in the nasal secretions ranged from 0.08 to 189.6 mu g ml(-1) (median 12.3 mu g ml(-1)); the ratio of the highest to the lowest concentrations was 2370, the difference between the 90th and 10th percentile concentrations was 189-fold and the difference between the 75th and 25th percentile values was 26. FSC concentrations were significantly lower in children aged less than or equal to 12 months (median 2.2 mu g ml(-1)) than in the older children (median 21.5 mu g ml(-1); p = 0.035). Between the first and the follow-up specimens, 65% of the children had greater than or equal to 2-fold difference in the levels of FSC in the secretions. Because an optimal standardization protein should show minimal variation between individuals and over time, FSC may not be a suitable protein for correction of the unknown dilution of nasopharyngeal specimens from children with upper respiratory tract infection.
Free secretory component (FSC) has been recommended as a reliable protein for correction of the unknown dilution in tracheal aspirate samples from preterm infants. To investigate whether FSC would also provide a valid standardization protein for samples of nasopharyngeal secretions, this study determined the intersubject variation and the alteration over time in the concentrations of FSC in nasal secretions from 35 children (median age 14 months) who participated in an antibiotic efficacy trial. Nasopharyngeal aspirates were obtained at enrolment and after 2-3 d. FSC in the specimens was quantified by a direct enzyme immunoassay. The concentrations of FSC in the nasal secretions ranged from 0.08 to 189.6 mu g ml(-1) (median 12.3 mu g ml(-1)); the ratio of the highest to the lowest concentrations was 2370, the difference between the 90th and 10th percentile concentrations was 189-fold and the difference between the 75th and 25th percentile values was 26. FSC concentrations were significantly lower in children aged less than or equal to 12 months (median 2.2 mu g ml(-1)) than in the older children (median 21.5 mu g ml(-1); p = 0.035). Between the first and the follow-up specimens, 65% of the children had greater than or equal to 2-fold difference in the levels of FSC in the secretions. Because an optimal standardization protein should show minimal variation between individuals and over time, FSC may not be a suitable protein for correction of the unknown dilution of nasopharyngeal specimens from children with upper respiratory tract infection.
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