A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Estimation of oxygen metabolism in a rat model of permanent ischemia using positron emission tomography with injectable O-15-O-2
Tekijät: Temma T, Magata Y, Kuge Y, Shimonaka S, Sano K, Katada Y, Kawashima H, Mukai T, Watabe H, Iida H, Saji H
Kustantaja: NATURE PUBLISHING GROUP
Julkaisuvuosi: 2006
Journal: Journal of Cerebral Blood Flow and Metabolism
Tietokannassa oleva lehden nimi: JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Lehden akronyymi: J CEREBR BLOOD F MET
Vuosikerta: 26
Numero: 12
Aloitussivu: 1577
Lopetussivu: 1583
Sivujen määrä: 7
ISSN: 0271-678X
DOI: https://doi.org/10.1038/sj.jcbfm.9600302
Tiivistelmä
The threshold of cerebral blood flow (CBF) into infarction in rats has been indicated to be similar to that in patients. However, CBF does not reflect metabolic function, and so estimations of oxygen metabolism have been required. Here, we estimated changes in oxygen metabolism after occluding the right middle cerebral artery (MCA) in rats using an injectable O-15-O-2 we developed. A decrease in CBF (left: 0.67 +/- 0.22 mL/min/g, right: 0.44 +/- 0.17 mL/min/g, P < 0.05) and compensatory increase in the oxygen extraction fraction (OEF) (left: 0.42 +/- 0.13, right: 0.50 +/- 0.19, P < 0.05) were observed at 1-h after occlusion. In contrast, a marked decrease in CBF and the cerebral metabolic rate for oxygen and a collapse of the compensatory OEF mechanism were found at 24 h after occlusion. Injectable O-15-O-2 could be used to reliably estimate oxygen metabolism in an infarction rat model with positron emission tomography.
The threshold of cerebral blood flow (CBF) into infarction in rats has been indicated to be similar to that in patients. However, CBF does not reflect metabolic function, and so estimations of oxygen metabolism have been required. Here, we estimated changes in oxygen metabolism after occluding the right middle cerebral artery (MCA) in rats using an injectable O-15-O-2 we developed. A decrease in CBF (left: 0.67 +/- 0.22 mL/min/g, right: 0.44 +/- 0.17 mL/min/g, P < 0.05) and compensatory increase in the oxygen extraction fraction (OEF) (left: 0.42 +/- 0.13, right: 0.50 +/- 0.19, P < 0.05) were observed at 1-h after occlusion. In contrast, a marked decrease in CBF and the cerebral metabolic rate for oxygen and a collapse of the compensatory OEF mechanism were found at 24 h after occlusion. Injectable O-15-O-2 could be used to reliably estimate oxygen metabolism in an infarction rat model with positron emission tomography.
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