The objective of this study is to introduce the usefulness of the liquid fingerprinting technology in spotting potential health hazards in foodstuff, exemplifying milk. The presented method relies on the use of long lifetime photoluminescent europium chelate and individual cross-reactive modulators in an array form. Here, we demonstrate the potency of liquid fingerprinting technology as a fast and cost-effective tool for detection of (i) melamine at the concentration of 1 ppm and (ii) Escherichia coli and Staphylococcus aureus at the level of 1000 and 3000 bacterial cells/ml, respectively, directly from milk without any bacterial concentration methods. The commonly used methods are often limited in their generic usability due to many factors, e.g., sensitivity, cost, and time of analysis. Presented technology offers monitoring within short measurement time and without any sample pre-treatment.