Separation of cells in density gradients with temporary solidification of the gradient: Application to Escherichia coli forms obtained by microwave treatment of pure cultures - UTU Research Portal

A1 Refereed original research article in a scientific journal

Separation of cells in density gradients with temporary solidification of the gradient: Application to Escherichia coli forms obtained by microwave treatment of pure cultures

Authors: Abashina T., Vainshtein M., Korpela S., Korpela T.

Publisher: Elsevier B.V.

Publication year: 2018

Journal:Journal of Microbiological Methods

Journal name in sourceJournal of Microbiological Methods

Volume: 153

First page : 45

Last page: 47

Number of pages: 3

ISSN: 0167-7012

eISSN: 1872-8359

DOI: https://doi.org/10.1016/j.mimet.2018.09.003

Abstract

Gelatin added in certain concentration into the density gradient mixtures allows to solidify and melt the gradient reversibly. Such a method was applied to separation of partially disintegrated Escherichia coli VKM B-125 cells in discontinuous sucrose gradient which was optimized to obtain small round “nano-cells” free from other cell components.

Gelatin added to the sucrose solutions had the advantages: i) accurate gradient layers were formed, ii) gelatin did not interfere with the cells and sub-particles during centrifugation, iii) solidified gelatin allowed to cut out the separated cell components, and iv) gelatin allowed to prepare ready-made solidified gradient centrifugation tubes into storage.