AtCYP20-2 is an auxiliary protein of the chloroplast NAD(P)H dehydrogenase complex

: Sirpio S, Holmstrom M, Battchikova N, Aro EM

Publisher: ELSEVIER SCIENCE BV

: 2009

: FEBS Letters

: FEBS LETTERS

: FEBS LETT

: 583

: 14

: 2355

: 2358

: 4

: 0014-5793

DOI: https://doi.org/10.1016/j.febslet.2009.06.031

AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it's co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants were studied, AtCYP20-2 was found to be strongly reduced especially in mutants deficient in the membrane domain of NDH, thus suggesting a role in the assembly of NDH hydrophobic domain. Lack of AtCYP20-2, however, did not lead to severe malfunction of NDH, indicating redundancy in the function of lumenal immunophilins. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.