A1 Refereed original research article in a scientific journal
Maximizing the hydrogen photoproduction yields in Chlamydomonas reinhardtii cultures: The effect of the H-2 partial pressure
Authors: Kosourov SN, Batyrova KA, Petushkova EP, Tsygankov AA, Ghirardi ML, Seibert M
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication year: 2012
Journal: International Journal of Hydrogen Energy
Journal name in source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Journal acronym: INT J HYDROGEN ENERG
Volume: 37
Issue: 10
First page : 8850
Last page: 8858
Number of pages: 9
ISSN: 0360-3199
DOI: https://doi.org/10.1016/j.ijhydene.2012.01.082
Photoproduction of H-2 gas has been examined in sulfur/phosphorus-deprived Chalmydomonas reinhardtii cultures, placed in photobioreactors (PhBRs) with different gas phase to liquid phase ratios (V-g.p/V-l.p). The results demonstrate that an increase in the ratio stimulates H-2 photoproduction activity in both algal suspension cultures and in algae entrapped in thin alginate films. In suspension cultures, a 4x increase (from similar to 0.5 to similar to 2) in V-g.p/V-l.p in a 2x increase (from 10.8 to 23.1 mmol l(-1) or 264-565 ml l(-1)) in the total yield of H-2 gas. Remarkably, 565 ml of H-2 gas per liter of the suspension culture is the highest yield ever reported for a wild-type strain in a time period of less than 190 h. In immobilized algae, where diffusion of H-2 from the medium to the PhBR gas phase is not affected by mixing, the maximum rate and yield of H-2 photoproduction occur in PhBRs with V-g.p/V-l.p above 7 or in a PhBR with smaller headspace, if the H-2 is effectively removed from the medium by continuous flushing of the headspace with argon. These experiments in combination with studies of the direct inhibitory effect of high H-2 concentrations in the PhBR headspace on H-2 photoproduction activity in algal cultures clearly show that H-2 photoproduction in algae depends significantly on the partial pressure of H-2 (not O-2 as previously thought) in the PhBR gas phase. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
Downloadable publication This is an electronic reprint of the original article. |  |
