A1 Refereed original research article in a scientific journal
Hydrogen production by cyanobacteria in an automated outdoor photobioreactor under aerobic conditions
Authors: Tsygankov AA, Fedorov AS, Kosourov SN, Rao KK
Publisher: WILEY
Publication year: 2002
Journal: Biotechnology and Bioengineering
Journal name in source: BIOTECHNOLOGY AND BIOENGINEERING
Journal acronym: BIOTECHNOL BIOENG
Volume: 80
Issue: 7
First page : 777
Last page: 783
Number of pages: 7
ISSN: 0006-3592
DOI: https://doi.org/10.1002/bit.10431
Abstract
The possibility of hydrogen production by a hydrogenase impaired mutant strain of Anabaena variabilis in outdoor culture was studied. A. computer-controlled rooftop (outdoor) tubular photobioreactor (4.35 L) was assembled. H-2 production rates by A. variabilis PK84 grown in CO2 + air in the photobioreactor were measured together with other parameters such as temperature, irradiance, pH, dry biomass weight, and pO(2), and Chl a concentrations during summer months of 1998 and 1999. Efficiencies of light energy bioconversion to H, energy and energy accumulated in biomass were calculated. The influence of irradiance, temperature, and mode of cultivation on H-2 production and efficiency of light energy bioconversion were evaluated. The culture produced up to 1.1 L H-2 day(-1) PhBR-1. The efficiency of light energy to H-2 energy bioconversion on some days was 0.094%. However, the conditions for maximum H-2 photoproduction and for maximum efficiency of light energy to H-2 energy bioconversion were not the same. A. variabilis PK84 could produce hydrogen for prolonged periods (up to 40 days) without injection of fresh inoculum. During this period photobioreactor produced 24.5 L of H-2. Possibilities for increasing the efficiency of light energy conversion are discussed. (C) 2002 Wiley Periodicals, Inc.
The possibility of hydrogen production by a hydrogenase impaired mutant strain of Anabaena variabilis in outdoor culture was studied. A. computer-controlled rooftop (outdoor) tubular photobioreactor (4.35 L) was assembled. H-2 production rates by A. variabilis PK84 grown in CO2 + air in the photobioreactor were measured together with other parameters such as temperature, irradiance, pH, dry biomass weight, and pO(2), and Chl a concentrations during summer months of 1998 and 1999. Efficiencies of light energy bioconversion to H, energy and energy accumulated in biomass were calculated. The influence of irradiance, temperature, and mode of cultivation on H-2 production and efficiency of light energy bioconversion were evaluated. The culture produced up to 1.1 L H-2 day(-1) PhBR-1. The efficiency of light energy to H-2 energy bioconversion on some days was 0.094%. However, the conditions for maximum H-2 photoproduction and for maximum efficiency of light energy to H-2 energy bioconversion were not the same. A. variabilis PK84 could produce hydrogen for prolonged periods (up to 40 days) without injection of fresh inoculum. During this period photobioreactor produced 24.5 L of H-2. Possibilities for increasing the efficiency of light energy conversion are discussed. (C) 2002 Wiley Periodicals, Inc.
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