A1 Refereed original research article in a scientific journal
Hydrogen photoproduction under continuous illumination by sulfur-deprived, synchronous Chlamydomonas reinhardtii cultures
Authors: Tsygankov A, Kosourov S, Seibert M, Ghirardi ML
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication year: 2002
Journal: International Journal of Hydrogen Energy
Journal name in source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Journal acronym: INT J HYDROGEN ENERG
Article number: PII S0360-3199(02)00108-8
Volume: 27
Issue: 11-12
First page : 1239
Last page: 1244
Number of pages: 6
ISSN: 0360-3199
DOI: https://doi.org/10.1016/S0360-3199(02)00108-8
Unsynchronized Chlamydomonas reinhardtii cells subsequently deprived of sulfur produce H-2 under continuous illumination in the laboratory for 3-4 days. However, cultures grown outdoors will be exposed to day-and-night cycles that may synchronize their growth and cell division. While it is clear that only insignificant amounts of H-2 can be produced by sulfur-deprived cells during the night period, little work has been done to examine the effects of the light/dark cycles preceding sulfur deprivation on subsequent H-2 photoproduction. We show that (a) C reinhardtii cells exhibit synchronized growth and cell division in the presence of acetate, (b) cells with the highest specific rates of H-2 photoproduction also have the highest rates of biomass accumulation, and (c) the highest rates of starch and protein degradation coincide with the highest rates of formate and acetate accumulation, but not with H-2 photoproduction. This work shows that it is possible to maximize the production of H-2 by sulfur-depriving synchronized cultures at about 4 h after the beginning of the light period.
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