A1 Refereed original research article in a scientific journal
Hydrogen Photoproduction by Nutrient-Deprived Chlamydomonas reinhardtii Cells Immobilized Within Thin Alginate Films Under Aerobic and Anaerobic Conditions
Authors: Kosourov SN, Seibert M
Publisher: JOHN WILEY & SONS INC
Publication year: 2009
Journal: Biotechnology and Bioengineering
Journal name in source: BIOTECHNOLOGY AND BIOENGINEERING
Journal acronym: BIOTECHNOL BIOENG
Volume: 102
Issue: 1
First page : 50
Last page: 58
Number of pages: 9
ISSN: 0006-3592
DOI: https://doi.org/10.1002/bit.22050
Abstract
A new technique for immobilizing H(2)-photoproducing green algae within a thin (<400 mu m) alginate film has been developed. Alginate films with entrapped sulfur/phosphorus-deprived Chlamydomonas reinhardtii, strain cc124, cells demonstrate (a) higher cell density (up to 2,000 mu g Chl mL(-1) of matrix), (b) kinetics of H(2) photoproduction similar to sulfur-deprived suspension cultures, (c) higher specific rates (up to 12.5 mu mol mg(-1) Chl h(-1)) of H(2) evolution, (d) light conversion efficiencies to H(2) of over 1% and (e) unexpectedly high resistance of the H(2)-photoproducing system to inactivation by atmospheric O(2). The algal cells, entrapped in alginate and then placed in vials containing 21% O(2) in the headspace, evolved up to 67% of the H(2) gas produced under anaerobic conditions. The results indicate that the lower susceptibility of the immobilized algal H(2)-producing system to inactivation by O(2) depends on two factors: (a) the presence of acetate in the medium, which supports higher rates of respiration and (b) the capability of the alginate polymer itself to effectively separate the entrapped cells from O(2) ill the liquid and headspace and restrict O(2) diffusion into the matrix. The strategy presented for immobilizing algal cells within thin polymeric matrices shows the potential for scale-up and possible future applications. Biotechnol. Bioeng. 2009;102: 50-58. (C) 2008 Wiley Periodicals, Inc.
A new technique for immobilizing H(2)-photoproducing green algae within a thin (<400 mu m) alginate film has been developed. Alginate films with entrapped sulfur/phosphorus-deprived Chlamydomonas reinhardtii, strain cc124, cells demonstrate (a) higher cell density (up to 2,000 mu g Chl mL(-1) of matrix), (b) kinetics of H(2) photoproduction similar to sulfur-deprived suspension cultures, (c) higher specific rates (up to 12.5 mu mol mg(-1) Chl h(-1)) of H(2) evolution, (d) light conversion efficiencies to H(2) of over 1% and (e) unexpectedly high resistance of the H(2)-photoproducing system to inactivation by atmospheric O(2). The algal cells, entrapped in alginate and then placed in vials containing 21% O(2) in the headspace, evolved up to 67% of the H(2) gas produced under anaerobic conditions. The results indicate that the lower susceptibility of the immobilized algal H(2)-producing system to inactivation by O(2) depends on two factors: (a) the presence of acetate in the medium, which supports higher rates of respiration and (b) the capability of the alginate polymer itself to effectively separate the entrapped cells from O(2) ill the liquid and headspace and restrict O(2) diffusion into the matrix. The strategy presented for immobilizing algal cells within thin polymeric matrices shows the potential for scale-up and possible future applications. Biotechnol. Bioeng. 2009;102: 50-58. (C) 2008 Wiley Periodicals, Inc.
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