A1 Refereed original research article in a scientific journal
Cultivating and harvesting of marine alga Nannochloropsis oculata in local municipal wastewater for biodiesel
Authors: Sema Şirin, Mika Sillanpää
Publisher: Elsevier Ltd
Publication year: 2015
Journal:Bioresource Technology
Journal name in sourceBioresource Technology
Volume: 191
First page : 79
Last page: 87
Number of pages: 9
ISSN: 18732976 09608524
DOI: https://doi.org/10.1016/j.biortech.2015.04.094
The feasibility of using the mixture of 
seawater and municipal wastewater; (1) the wastewater before activated 
sludge tank, just after primary settling (BAS) and (2) the wastewater 
after activated sludge tank, just before addition of polymer flocculants
 (AAS); as culture medium for the cultivation of marine microalgae Nannochloropsis oculata was investigated.
10%
 BAS, 20% BAS and 10% AAS, 20% AAS, 50% AAS, 70% AAS, 100% AAS effluent 
loadings were well adapted to used wastewater. Sufficient dry weights 
obtained (345–406 mg L−1) with growth rates 0.37–0.45 for 
aerated cultures. High TN and TP removals (∼74–90%) were achieved. 
Harvesting technique for grown cultures was also studied with natural 
sedimentation and pH induced flocculation. By alkalinity induced 
flocculation, at pH values of 10.50, high recovery of the cells (∼80%) 
achieved with high sedimentation rates in 10 min. The flocculation efficiencies decreased, sedimentation rates increased with the increase of the cell concentration.