A1 Refereed original research article in a scientific journal
Recombinant luminescent bacteria for measuring bioavailable arsenite and antimonite
Authors: Tauriainen S, Karp H, Chang W, Virta M
Publisher: AMER SOC MICROBIOLOGY
Publication year: 1997
Journal: Applied and Environmental Microbiology
Journal name in source: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Journal acronym: APPL ENVIRON MICROB
Volume: 63
Issue: 11
First page : 4456
Last page: 4461
Number of pages: 6
ISSN: 0099-2240
Abstract
Luminescent bacterial strains for the measurement of bioavailable arsenite and antimony were constructed, The expression of firefly luciferase was controlled by the regulatory unit of the ars operon of Staphylococcus aureus plasmid pI258 in recombinant plasmid pT0021, with S. aureus RN4220, Bacillus subtilis BR151, and Escherichia coli MC1061 as host strains, Strain RN4220(pT0021) was found to be the most sensitive for metal detection responding to arsenite, antimonite, and cadmium, the lowest detectable concentrations being 100, 33, and 330 nhl, respectively, Strains BR151(pT0021) and MC1061(pT0021) responded to arsenite, arsenate, antimonite, and cadmium, the lowest detectable concentrations being 3.3 and 330 mu M and 330 and 330 nM with BR151(pT0021), respectively, and 3.3, 33, 3.3, and 33 CIM with MC1061(pT0021), respectively, In the absence of the mentioned ions, the expression of luciferase was repressed and only a small amount of background light was emitted, Other ions did not notably interfere with the measurement in any of the strains tested, Freeze-drying of the cells did not decrease the sensitivity of the detection of arsenite; however, the induction coefficients were somewhat lower.
Luminescent bacterial strains for the measurement of bioavailable arsenite and antimony were constructed, The expression of firefly luciferase was controlled by the regulatory unit of the ars operon of Staphylococcus aureus plasmid pI258 in recombinant plasmid pT0021, with S. aureus RN4220, Bacillus subtilis BR151, and Escherichia coli MC1061 as host strains, Strain RN4220(pT0021) was found to be the most sensitive for metal detection responding to arsenite, antimonite, and cadmium, the lowest detectable concentrations being 100, 33, and 330 nhl, respectively, Strains BR151(pT0021) and MC1061(pT0021) responded to arsenite, arsenate, antimonite, and cadmium, the lowest detectable concentrations being 3.3 and 330 mu M and 330 and 330 nM with BR151(pT0021), respectively, and 3.3, 33, 3.3, and 33 CIM with MC1061(pT0021), respectively, In the absence of the mentioned ions, the expression of luciferase was repressed and only a small amount of background light was emitted, Other ions did not notably interfere with the measurement in any of the strains tested, Freeze-drying of the cells did not decrease the sensitivity of the detection of arsenite; however, the induction coefficients were somewhat lower.
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