B1 Non-refereed article in a scientific journal
Base and sequence selective cleavage of RNA phosphodiester bonds by Zn(II) azacrown chelates
Authors: Wang Q, Jancso A, Niittymaki T, Poijarvi-Virta P, Ketomaki K, Virta P, Leino E, Szilagyi I, Mikkola S, Gajda T, Lonnberg H
Publication year: 2008
Journal: Collection Symposium Series
Journal name in source: CHEMISTRY OF NUCLEIC ACID COMPONENTS
Journal acronym: COLL SYMPOS SERIES
Volume: 10
First page : 63
Last page: 70
Number of pages: 8
ISBN: 978-80-86241-29-6
Abstract
Zn2+ chelates of azacrowns exhibit two interesting properties: they promote the cleavage of RNA phosphodiester bonds and undergo selective binding to nucleic acid bases, the affinity decreasing in the order Ura > Gua > Ade approximate to Cyt. Binding of di- and trinuclear Zn2+ azacrown chelates to contiguous bases in RNA is a co-operative process. The stability of a ternary complex may, in fact, be so high that the complex formation is possible at intracellular concentrations of Zn2+. Various di- and trinucleating azacrown ligands have been prepared and studied as base-selective cleaving agents of RNA. Sequence-selective artificial ribonucleases have, in turn, been obtained by tethering azacrown ligands to a 2'-O-methyl oligoribonucleotide that recognizes the target sequence. The results of these studies are surveyed.
Zn2+ chelates of azacrowns exhibit two interesting properties: they promote the cleavage of RNA phosphodiester bonds and undergo selective binding to nucleic acid bases, the affinity decreasing in the order Ura > Gua > Ade approximate to Cyt. Binding of di- and trinuclear Zn2+ azacrown chelates to contiguous bases in RNA is a co-operative process. The stability of a ternary complex may, in fact, be so high that the complex formation is possible at intracellular concentrations of Zn2+. Various di- and trinucleating azacrown ligands have been prepared and studied as base-selective cleaving agents of RNA. Sequence-selective artificial ribonucleases have, in turn, been obtained by tethering azacrown ligands to a 2'-O-methyl oligoribonucleotide that recognizes the target sequence. The results of these studies are surveyed.
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