A1 Refereed original research article in a scientific journal
FLUORESCENCE AND ABSORPTION SPECTROSCOPIC PROPERTIES OF RNA 5'-CAP ANALOGS DERIVED FROM 7-METHYL-GUANOSINES, N-2,7-DIMETHYL-GUANOSINES AND N-2,N-2,7-TRIMETHYL-GUANOSINES
Authors: WIECZOREK Z, STEPINSKI J, JANKOWSKA M, LONNBERG H
Publisher: ELSEVIER SCIENCE SA LAUSANNE
Publication year: 1995
Journal:: Journal of Photochemistry and Photobiology B: Biology
Journal name in source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Journal acronym: J PHOTOCH PHOTOBIO B
Volume: 28
Issue: 1
First page : 57
Last page: 63
Number of pages: 7
ISSN: 1011-1344
DOI: https://doi.org/10.1016/1011-1344(94)07098-9
Abstract
Absorption and fluorescence properties of several cap analogues, namely nucleosides, nucleoside 5'-monophosphates and P-1,P-3-dinucleoside triphosphates derived from 7-methylguanine, N-2,7-dimethylguanine and N-2,N-2,7-trimethylguanine, have been studied. The data obtained include the absorption and fluorescence spectra of the cationic (N1-protonated) and zwitterionic (N1-deprotonated) species, the pK(a) values of the ground and excited states of the methylated base moiety and the effect of temperature and solvent composition (mixtures of water and 1,4-dioxane) on the fluorescence intensity. Furthermore, the fluorescence lifetimes of N-2,N-2,7-trimethylguanosine 5'-triphosphate and P-1-guanosine(5')-P-3-[N-2,N-2,7-trimethylguanosine(5')] triphosphate have been determined as a function of temperature. These data dearly indicate that dynamic quenching must be taken into account when the extent of the intramolecular stacking of the latter compound is estimated by fluorescence spectroscopy.
Absorption and fluorescence properties of several cap analogues, namely nucleosides, nucleoside 5'-monophosphates and P-1,P-3-dinucleoside triphosphates derived from 7-methylguanine, N-2,7-dimethylguanine and N-2,N-2,7-trimethylguanine, have been studied. The data obtained include the absorption and fluorescence spectra of the cationic (N1-protonated) and zwitterionic (N1-deprotonated) species, the pK(a) values of the ground and excited states of the methylated base moiety and the effect of temperature and solvent composition (mixtures of water and 1,4-dioxane) on the fluorescence intensity. Furthermore, the fluorescence lifetimes of N-2,N-2,7-trimethylguanosine 5'-triphosphate and P-1-guanosine(5')-P-3-[N-2,N-2,7-trimethylguanosine(5')] triphosphate have been determined as a function of temperature. These data dearly indicate that dynamic quenching must be taken into account when the extent of the intramolecular stacking of the latter compound is estimated by fluorescence spectroscopy.
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