A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Chemical Composition and Bioactivity of Pleiogynium timorense (Anacardiaceae)
Tekijät: Al Sayed E, Martiskainen O, Sinkkonen J, Pihlaja K, Ayoub N, Singab AEN, El-Azizi M
Kustantaja: NATURAL PRODUCTS INC
Julkaisuvuosi: 2010
Journal: Natural Product Communications
Tietokannassa oleva lehden nimi: NATURAL PRODUCT COMMUNICATIONS
Lehden akronyymi: NAT PROD COMMUN
Numero sarjassa: 4
Vuosikerta: 5
Numero: 4
Aloitussivu: 545
Lopetussivu: 550
Sivujen määrä: 6
ISSN: 1934-578X
Tiivistelmä
A liquid chromatography-diode array detection-electrospray ionization mass spectrometric (HPLC-PDA-ESI/MS/MS) method was used for the analysis of the phenolic composition of the cthanolic extract obtained from the leaves of Pleiogynium timorense (DC.) Leenh. Twenty compounds were detected and tentatively characterized. In addition, further phytochemical investigations of the extract resulted in the isolation of twelve major phenolic compounds. Evidence of the structures of these compounds was obtained based on the interpretation of the UV, (1)H NMR, (13)C NMR and DQF-COSY spectral data. The antioxidant effect of the ethanolic extract was examined in vitro using 1,1-diphenyl-2-picrylhydrazyl (DPPH.) and super oxide anion radical scavenging assays. DPPH radical scavenging activity was observed for the extract, with an IC(50) of 21.9 mu s/mL, while its super oxide anion scavenging activity was less pronounced, with an IC(50) of 123.5 mu g/mL The ethanolic extract showed significant hypoglycemic, antioxidant and anti-inflammatory properties. This study suggests that the ethanolic extract of Pleiogynium timorense is a potential source of antioxidant compounds, relatively non-toxic, and have possible beneficial health effects.
A liquid chromatography-diode array detection-electrospray ionization mass spectrometric (HPLC-PDA-ESI/MS/MS) method was used for the analysis of the phenolic composition of the cthanolic extract obtained from the leaves of Pleiogynium timorense (DC.) Leenh. Twenty compounds were detected and tentatively characterized. In addition, further phytochemical investigations of the extract resulted in the isolation of twelve major phenolic compounds. Evidence of the structures of these compounds was obtained based on the interpretation of the UV, (1)H NMR, (13)C NMR and DQF-COSY spectral data. The antioxidant effect of the ethanolic extract was examined in vitro using 1,1-diphenyl-2-picrylhydrazyl (DPPH.) and super oxide anion radical scavenging assays. DPPH radical scavenging activity was observed for the extract, with an IC(50) of 21.9 mu s/mL, while its super oxide anion scavenging activity was less pronounced, with an IC(50) of 123.5 mu g/mL The ethanolic extract showed significant hypoglycemic, antioxidant and anti-inflammatory properties. This study suggests that the ethanolic extract of Pleiogynium timorense is a potential source of antioxidant compounds, relatively non-toxic, and have possible beneficial health effects.
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