A2 Vertaisarvioitu katsausartikkeli tieteellisessä lehdessä
Functional genomics in aquatic toxicology-Do not forget the function
Tekijät: Nikinmaa M, Rytkonen KT
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 2011
Journal: Aquatic Toxicology
Tietokannassa oleva lehden nimi: AQUATIC TOXICOLOGY
Lehden akronyymi: AQUAT TOXICOL
Vuosikerta: 105
Aloitussivu: 16
Lopetussivu: 24
Sivujen määrä: 9
ISSN: 0166-445X
DOI: https://doi.org/10.1016/j.aquatox.2011.05.019
Tiivistelmä
Toxicological responses of an organism are disturbances of function. This as a starting point we review and discuss issues that we consider important in applying functional genomics to aquatic toxicology. Functional genomics includes all the steps in gene expression pathway. Thus, ultimately the goal is to relate genome information to protein activity. In ecotoxicogenomics the toxicological responses must further be combined with responses to natural environmental changes. We focus on fish, but also consider commonly used invertebrates, mainly Daphnia. We first go through the toxicologically important features of genomes of aquatic animals, and then review the reference gene approach to quantify transcript amount. Thereafter we emphasize the need to relate the mRNA and protein levels, and protein activity of individual genes. Finally we discuss how functional genomic investigations may be important in resolving current environmental problems and give our views of valuable future research topics. (C) 2011 Elsevier B.V. All rights reserved.
Toxicological responses of an organism are disturbances of function. This as a starting point we review and discuss issues that we consider important in applying functional genomics to aquatic toxicology. Functional genomics includes all the steps in gene expression pathway. Thus, ultimately the goal is to relate genome information to protein activity. In ecotoxicogenomics the toxicological responses must further be combined with responses to natural environmental changes. We focus on fish, but also consider commonly used invertebrates, mainly Daphnia. We first go through the toxicologically important features of genomes of aquatic animals, and then review the reference gene approach to quantify transcript amount. Thereafter we emphasize the need to relate the mRNA and protein levels, and protein activity of individual genes. Finally we discuss how functional genomic investigations may be important in resolving current environmental problems and give our views of valuable future research topics. (C) 2011 Elsevier B.V. All rights reserved.
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