A1 Refereed original research article in a scientific journal

Characterisation of polymorphic microsatellite loci for the bryozoan Fredericella sultana, the primary host of the causative agent of salmonid proliferative kidney disease




AuthorsDmitry Filippenko, Hanna Hartikainen, Beth Okamura, Anti Vasemägi

PublisherSPRINGER

Publication year2014

JournalConservation Genetics Resources

Journal name in sourceCONSERVATION GENETICS RESOURCES

Journal acronymCONSERV GENET RESOUR

Volume6

Issue2

First page 481

Last page482

Number of pages2

ISSN1877-7252

DOIhttps://doi.org/10.1007/s12686-013-0133-0(external)


Abstract

The freshwater bryozoan Fredericella sultana is the most common primary host for the myxozoan parasite Tetracapsuloides bryosalmonae, causing proliferative kidney disease (PKD) in salmonid fish. An Illumina next-generation sequencing library containing genomic DNA from both T. bryosalmonae and F. sultana yielded 10,653 microsatellite motifs. Twenty-four loci were tested in both species and 12 novel polymorphic tri- and tetranucleotide microsatellite markers were developed for F. sultana. These markers provide a valuable resource for population genetic studies of F. sultana, whilst the apparent lack of microsatellites in the PKD agent suggests a low frequency of tri- and tetranucleotide repeats in the T. bryosalmonae genome.




Last updated on 2024-26-11 at 13:28