A1 Refereed original research article in a scientific journal
Characterisation of polymorphic microsatellite loci for the bryozoan Fredericella sultana, the primary host of the causative agent of salmonid proliferative kidney disease
Authors: Dmitry Filippenko, Hanna Hartikainen, Beth Okamura, Anti Vasemägi
Publisher: SPRINGER
Publication year: 2014
Journal: Conservation Genetics Resources
Journal name in source: CONSERVATION GENETICS RESOURCES
Journal acronym: CONSERV GENET RESOUR
Volume: 6
Issue: 2
First page : 481
Last page: 482
Number of pages: 2
ISSN: 1877-7252
DOI: https://doi.org/10.1007/s12686-013-0133-0(external)
The freshwater bryozoan Fredericella sultana is the most common primary host for the myxozoan parasite Tetracapsuloides bryosalmonae, causing proliferative kidney disease (PKD) in salmonid fish. An Illumina next-generation sequencing library containing genomic DNA from both T. bryosalmonae and F. sultana yielded 10,653 microsatellite motifs. Twenty-four loci were tested in both species and 12 novel polymorphic tri- and tetranucleotide microsatellite markers were developed for F. sultana. These markers provide a valuable resource for population genetic studies of F. sultana, whilst the apparent lack of microsatellites in the PKD agent suggests a low frequency of tri- and tetranucleotide repeats in the T. bryosalmonae genome.