A1 Refereed original research article in a scientific journal
Ultra high performance liquid chromatography-mass spectrometric analysis of oxidized free fatty acids and acylglycerols
Authors: Tarvainen M, Suomela JP, Kallio H
Publisher: WILEY-BLACKWELL
Publication year: 2011
Journal: European Journal of Lipid Science and Technology
Journal name in source: EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Journal acronym: EUR J LIPID SCI TECH
Number in series: 4
Volume: 113
Issue: 4
First page : 409
Last page: 422
Number of pages: 14
ISSN: 1438-7697
DOI: https://doi.org/10.1002/ejlt.201000454
Abstract
A fast ultra high performance liquid chromatography (UHPLC)-electrospray ionization (ESI)-mass spectrometric (MS) method was developed for simultaneous analysis of free fatty acids (FFAs), monoacylglycerols (MAG), diacylglycerols (DAG), triacylglycerols (TAG), and their oxidized equivalents. Effect of elevated column temperature was studied in order to optimize the chromatography of closely eluting peaks and to reduce high back pressure formed in UHPLC. The elevated temperature enabled high flow rate, better mass transfer, and therefore more narrow peaks and better separation of the analytes. The new method was applied to the analysis of total lipid extracts of lipolysis samples prepared by an artificial digestion model in order to investigate oxidized lipids and changes in their profiles in the chyme. Over 150 compounds were identified from the extracts. The UHPLC-ESI-MS method was proved to be fast, highly selective, and sensitive. Compared to a previously used high performance LC-ESI-MS method, the new UHPLC-ESI-MS method was over five times faster and consumed one tenth of the solvents while producing comparable quantitative results.
A fast ultra high performance liquid chromatography (UHPLC)-electrospray ionization (ESI)-mass spectrometric (MS) method was developed for simultaneous analysis of free fatty acids (FFAs), monoacylglycerols (MAG), diacylglycerols (DAG), triacylglycerols (TAG), and their oxidized equivalents. Effect of elevated column temperature was studied in order to optimize the chromatography of closely eluting peaks and to reduce high back pressure formed in UHPLC. The elevated temperature enabled high flow rate, better mass transfer, and therefore more narrow peaks and better separation of the analytes. The new method was applied to the analysis of total lipid extracts of lipolysis samples prepared by an artificial digestion model in order to investigate oxidized lipids and changes in their profiles in the chyme. Over 150 compounds were identified from the extracts. The UHPLC-ESI-MS method was proved to be fast, highly selective, and sensitive. Compared to a previously used high performance LC-ESI-MS method, the new UHPLC-ESI-MS method was over five times faster and consumed one tenth of the solvents while producing comparable quantitative results.
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