Refereed journal article or data article (A1)

Isolation and identification of cyclic lipopeptides from Paenibacillus ehimensis, strain IB-X-b




List of Authors: Gleb Aktuganov, Jouni Jokela, Henri Kivelä, Elvira Khalikova, Alexander Melentjev, Nailia Galimzianova, Lyudmila Kuzmina, Petri Kouvonen, Juha-Pekka Himanen, Petri Susi, Timo Korpela

Publisher: Elsevier B.V.

Publication year: 2014

Journal: Journal of Chromatography B

Journal name in source: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Journal acronym: J Chromatogr B Analyt Technol Biomed Life Sci

Volume number: 973C

Issue number: 973

Number of pages: 8

ISSN: 1873-376X

eISSN: 1570-0232

DOI: http://dx.doi.org/10.1016/j.jchromb.2014.09.042


Abstract

Antifungal lipopeptides produced by an antagonistic bacterium, Paenibacillus ehimensis strain IB-X-b, were purified and analyzed. The acetone extract of the culture supernatant contained an antifungal amphiphilic fraction stainable with ninhydrin on thin layer chromatography. The fraction was further purified with water-methanol extraction followed by a chromatography on a C18-support. The analysis with LC-MS showed presence of two main series of homologous compounds, family of depsipeptides containing a hydroxy fatty acid, three 2,4-diaminobutyric acid (Dab) residues, five hydrophobic amino acids and one Ser/Thr residue, and cyclic lipopeptides of bacillomycin L and fengycin/plipastatin/agrastatin families. The prevailing compounds in this group are bacillomycin L-C15, fengycin/plipastatin A-C16 together with their homologues responsible for the majority of fungal growth inhibition by P. ehimensis IB-X-b.



Last updated on 2021-24-06 at 08:26