A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Significance of the transient receptor potential canonical 2 (TRPC2) channel in the regulation of rat thyroid FRTL-5 cell proliferation, migration, adhesion and invasion
Tekijät: Sukumaran P, Löf C, Pulli I, Kemppainen K, Viitanen T, Törnquist K
Kustantaja: ELSEVIER IRELAND LTD
Julkaisuvuosi: 2013
Lehti:: Molecular and Cellular Endocrinology
Tietokannassa oleva lehden nimi: MOLECULAR AND CELLULAR ENDOCRINOLOGY
Lehden akronyymi: MOL CELL ENDOCRINOL
Numero sarjassa: 1-2
Vuosikerta: 374
Numero: 1-2
Aloitussivu: 10
Lopetussivu: 21
Sivujen määrä: 12
ISSN: 0303-7207
DOI: https://doi.org/10.1016/j.mce.2013.03.026
Tiivistelmä
Mammalian transient receptor potential (TRP) channels are involved in many physiologically important processes. Here, we have studied the significance of the TRPC2 channel in the regulation of rat thyroid FRTL-5 cell proliferation, migration, adhesion and invasion, using stable TRPC2 (shTRPC2) knock-down cells. In the shTRPC2 cells, proliferation was decreased due to a prolonged G1/S cell cycle phase. The tumor suppressor p53 and the cyclin-dependant kinase inhibitors p27 and p21 were upregulated. Cell invasion, adhesion and migration were also attenuated in shTRPC2 cells, probably due to decreased activity of both Rac and calpain, and a decreased secretion and activity of matrix metalloproteinase 2. The. attenuated proliferation, migration, invasion and ATP-evoked calcium entry was mimicked by over-expressing a non-conducting, truncated TRPC2 (TRPC2-DN) in wild type cells, and was reversed by overexpression of TRPC2-GFP in shTRPC2 cells. In conclusion, TRPC2 is an important regulator of rat thyroid. cell function. (c) 2013 Elsevier Ireland Ltd. All rights reserved.
Mammalian transient receptor potential (TRP) channels are involved in many physiologically important processes. Here, we have studied the significance of the TRPC2 channel in the regulation of rat thyroid FRTL-5 cell proliferation, migration, adhesion and invasion, using stable TRPC2 (shTRPC2) knock-down cells. In the shTRPC2 cells, proliferation was decreased due to a prolonged G1/S cell cycle phase. The tumor suppressor p53 and the cyclin-dependant kinase inhibitors p27 and p21 were upregulated. Cell invasion, adhesion and migration were also attenuated in shTRPC2 cells, probably due to decreased activity of both Rac and calpain, and a decreased secretion and activity of matrix metalloproteinase 2. The. attenuated proliferation, migration, invasion and ATP-evoked calcium entry was mimicked by over-expressing a non-conducting, truncated TRPC2 (TRPC2-DN) in wild type cells, and was reversed by overexpression of TRPC2-GFP in shTRPC2 cells. In conclusion, TRPC2 is an important regulator of rat thyroid. cell function. (c) 2013 Elsevier Ireland Ltd. All rights reserved.
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