A1 Refereed original research article in a scientific journal
Inhibitory effect of curcuminoid pretreatments on endogenous dentin proteases
Authors: Roda Seseogullari-Dirihan, Meltem Tekbas Atay, David Henry Pashley, Arzu Tezvergil-Mutluay
Publisher: JAPANESE SOC DENTAL MATERIALS DEVICES
Publication year: 2018
Journal: Dental Materials Journal
Journal name in source: DENTAL MATERIALS JOURNAL
Journal acronym: DENT MATER J
Volume: 37
Issue: 3
First page : 445
Last page: 452
Number of pages: 8
ISSN: 0287-4547
eISSN: 1881-1361
DOI: https://doi.org/10.4012/dmj.2017-116
Abstract
The aim of this study was to evaluate the effect of curcuminoids on the dentin endogenous protease activity. Demineralized dentin were pretreated with 50 or 100 0/1 of three different curcuminoids for 60 s and incubated up to 3 months. Untreated beams served as controls. Dry dentin mass was measured after incubation. Aliquots were analyzed for the quantity of ICTP and CTX releases for MMP and cathepsin-K mediated degradation, respectively. The effect of curcuminoids on matrix-bound MMP and soluble rhIVIMP-9 were measured using an activity assay. Data were subjected to repeated-measures-ANOVA (alpha=0.05). Gelatinolytic activity was analyzed using zymography. ICTP and CTX release and dry mass loss of curcuminoid-treated groups were significantly lower than the control. Inhibition of rhMMP-9 varied from 29-49% among curcumonoid-treated groups, whereas no inhibition was observed at untreated control (p>0.05). Results were confirmed by zymography. The study showed that the pretreatment of dentin matrices by curcuminoids decreases endogenous protease activity-mediated degradation in dentin.
The aim of this study was to evaluate the effect of curcuminoids on the dentin endogenous protease activity. Demineralized dentin were pretreated with 50 or 100 0/1 of three different curcuminoids for 60 s and incubated up to 3 months. Untreated beams served as controls. Dry dentin mass was measured after incubation. Aliquots were analyzed for the quantity of ICTP and CTX releases for MMP and cathepsin-K mediated degradation, respectively. The effect of curcuminoids on matrix-bound MMP and soluble rhIVIMP-9 were measured using an activity assay. Data were subjected to repeated-measures-ANOVA (alpha=0.05). Gelatinolytic activity was analyzed using zymography. ICTP and CTX release and dry mass loss of curcuminoid-treated groups were significantly lower than the control. Inhibition of rhMMP-9 varied from 29-49% among curcumonoid-treated groups, whereas no inhibition was observed at untreated control (p>0.05). Results were confirmed by zymography. The study showed that the pretreatment of dentin matrices by curcuminoids decreases endogenous protease activity-mediated degradation in dentin.
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