Refereed journal article or data article (A1)

Loss-of-function of IFT88 determines metabolic phenotypes in thyroid cancer

List of Authors: Junguee Lee, Shinae Yi, Minho Won, Young Shin Song, Hyon-Seung Yi, Young Joo Park, Ki Cheol Park,
Jung Tae Kim, Joon Young Chang, Min Joung Lee, Hae Joung Sul, Ji Eun Choi, Koon Soon Kim, Jukka Kero, Joon Kim, Minho Shong

Publisher: Nature Publishing Group

Publication year: 2018

Journal: Oncogene

Journal name in source: Oncogene

Volume number: 37

Issue number: 32

Start page: 4455

End page: 4474

Number of pages: 20

ISSN: 0950-9232

eISSN: 1476-5594



Primary cilia are microtubule-based, dynamic organelles characterized by
continuous assembly and disassembly. The intraflagellar transport (IFT)
machinery, including IFT88 in cilia, is involved in the maintenance of
bidirectional motility along the axonemes, which is required for
ciliogenesis and functional competence. Cancer cells are frequently
associated with loss of primary cilia and IFT functions. However, there
is little information on the role of IFT88 or primary cilia in the
metabolic remodeling of cancer cells. Therefore, we investigated the
cellular and metabolic effects of the loss-of-function (LOF) mutations
of IFT88/primary cilia in thyroid cancer cells. IFT88-deficient
8505C thyroid cancer cells were generated using the CRISPR/Cas9 system,
and RNA-sequencing analysis was performed. LOF of the IFT88 gene resulted in a marked defect in ciliogenesis and mitochondrial oxidative function. Gene expression patterns in IFT88-deficient thyroid cancer cells favored glycolysis and lipid biosynthesis. However, LOF of IFT88/primary cilia did not promote thyroid cancer cell proliferation, migration, and invasion. The results suggest that IFT88/primary cilia play a role in metabolic reprogramming in thyroid cancer cells.

Last updated on 2021-24-06 at 10:43