A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Genome-wide Profiling of Interleukin-4 and STAT6 Transcription Factor Regulation of Human Th2 Cell Programming
Tekijät: Elo Laura L, Järvenpää Henna, Tuomela Soile, Raghav Sunil, Ahlfors Helena, Laurila Kirsti, Gupta Bhawna, Lund Riikka J, Tahvanainen Johanna, Hawkins David R, Oresic Matej, Lähdesmäki Harri, Rasool Omid, Rao Kanury V, Aittokallio Tero, Lahesmaa Riitta
Kustantaja: CELL PRESS
Julkaisuvuosi: 2010
Journal: Immunity
Tietokannassa oleva lehden nimi: IMMUNITY
Lehden akronyymi: IMMUNITY
Numero sarjassa: 6
Vuosikerta: 32
Numero: 6
Aloitussivu: 852
Lopetussivu: 862
Sivujen määrä: 11
ISSN: 1074-7613
DOI: https://doi.org/10.1016/j.immuni.2010.06.011
Tiivistelmä
Dissecting the molecular mechanisms by which T helper (Th) cells differentiate to effector Th2 cells is important for understanding the pathogenesis of immune-mediated diseases, such as asthma and allergy. Because the STAT6 transcription factor is an upstream mediator required for interleukin-4 (IL-4)-induced Th2 cell differentiation, its targets include genes important for this process. Using primary human CD4(+) T cells, and by blocking STAT6 with RNAi, we identified a number of direct and indirect targets of STAT6 with ChIP sequencing. The integration of these data sets with detailed kinetics of IL-4-driven transcriptional changes showed that STAT6 was predominantly needed for the activation of transcription leading to the Th2 cell phenotype. This integrated genome-wide data on IL-4- and STAT6-mediated transcription provide a unique resource for studies on Th cell differentiation and, in particular, for designing interventions of human Th2 cell responses.
Dissecting the molecular mechanisms by which T helper (Th) cells differentiate to effector Th2 cells is important for understanding the pathogenesis of immune-mediated diseases, such as asthma and allergy. Because the STAT6 transcription factor is an upstream mediator required for interleukin-4 (IL-4)-induced Th2 cell differentiation, its targets include genes important for this process. Using primary human CD4(+) T cells, and by blocking STAT6 with RNAi, we identified a number of direct and indirect targets of STAT6 with ChIP sequencing. The integration of these data sets with detailed kinetics of IL-4-driven transcriptional changes showed that STAT6 was predominantly needed for the activation of transcription leading to the Th2 cell phenotype. This integrated genome-wide data on IL-4- and STAT6-mediated transcription provide a unique resource for studies on Th cell differentiation and, in particular, for designing interventions of human Th2 cell responses.
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