B3 Vertaisarvioimaton artikkeli konferenssijulkaisussa
Cu2+ dependent and spontaneous cleavage of mRNA 5 '-cap structure
Tekijät: Mikkola S
Julkaisuvuosi: 2008
Tietokannassa oleva lehden nimi: CHEMISTRY OF NUCLEIC ACID COMPONENTS
Lehden akronyymi: COLL SYMPOS SERIES
Vuosikerta: 10
Aloitussivu: 410
Lopetussivu: 411
Sivujen määrä: 2
ISBN: 978-80-86241-29-6
Tiivistelmä
The cleavage of mRNA 5'-cap structure has been studied under neutral conditions in the presence and in the absence of Cu2+ complexes. Cu2+ complexes efficiently promote the cleavage reaction, which, most likely, proceeds via the hydrolysis of the triphosphate bridge. In the absence of the metal ion catalyst, the reaction is slower, and the pathway is different. Initially, the m(7)Gua base is cleaved, which results in the hydrolysis of the N-glycosidic bond and, subsequently, the cleavage of the phosphate bridge.
The cleavage of mRNA 5'-cap structure has been studied under neutral conditions in the presence and in the absence of Cu2+ complexes. Cu2+ complexes efficiently promote the cleavage reaction, which, most likely, proceeds via the hydrolysis of the triphosphate bridge. In the absence of the metal ion catalyst, the reaction is slower, and the pathway is different. Initially, the m(7)Gua base is cleaved, which results in the hydrolysis of the N-glycosidic bond and, subsequently, the cleavage of the phosphate bridge.
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